Absolute versus relative quantification |
Advantages of PerfeCTa™ qPCR FastMix™over PerfeCTa™ qPCR SuperMix |
Applications for the qPCR FastMix |
cDNA input in SYBR and Taqman qPCR |
Compatibility of Quanta Biosciences reagents with Roche’s LightCycler 1.5/2.0 |
Difference between AccuFast™ Taq DNA polymerase and AccuStart™ Taq DNA Polymerase |
Expiration date |
Fast cycling reaction volume |
Improving qPCR specificity |
Information to provide when calling technical support |
Instrument Compatibility- SYBR Green Detection |
Instrument Compatibility-Taqman Probe Detection |
Is the PerfeCTa™ qPCR FastMix™ offered with UNG? |
Mixing PCR reactions |
Multiplexing |
One-step or Two-step reaction? |
Performance of PerfeCTa™ qPCR FastMix compared with PerfeCTa™ qPCR Supermix |
Primer quality/sequence effect on qPCR effeciency |
Primer validation/quality |
Replicates |
Sensitivity of SYBR and Taqman reagents chemistries |
Stability at room temperature |
Using PerfeCTa™ qPCR SuperMix in Fast mode |
What component in the qPCR FastMix allows rapid amplification results? |