sparQ RNA-Seq HMR Kit
Ultra FAST RNA library prep with integrated rRNA & globin depletion
sparQ RNA-Seq HMR Kit simultaneously depletes rRNA and globin mRNA while generating stranded RNA-seq libraries for Illumina® NGS platforms in 5 hours. In a single step and tube, RNA fragmentation and depletion of abundant ribosomal and globin transcripts (human, mouse, and rat) are integrated. The proprietary, highly optimized enzymes and streamlined workflow generate high quality, directional transcriptome NGS libraries from either intact or degraded RNA samples, with key improvements for low input and FFPE samples.
Features & Benefits
- High quality directional RNA library prep in 5 hours
- Simple workflow with 3 reaction tubes, 9 steps and 9 components
- Efficient and integrated removal of rRNA and globin mRNA from
human/mouse/rat (HMR) samples
Improved results for samples with limited quantity and/or poor quality RNA
sparQ RNA-Seq HMR Kit is intended for molecular biology applications. This product is not intended for the diagnosis, prevention or treatment of a disease.
sparQ UDI Adapter Resources
- Technical Note – Setting up sparQ Library Prep Kit in Illumina Experiment Manager
- Illumina Experiment Manager version 1.8 files (zip-download)
- Illumina Experiment Manager version 1.9 files (zip-download)
- sparQ UDI Adapter sequence overview
Storage and Handling:
- Store the product in a constant temperature freezer at -25°C to -15°C upon receipt.
- Once the ‘UDI Dilution Buffer’ has been thawed for use, it should be stored at 2 – 8°C.
- For lot specific expiry date, refer to package label, Certificate of Analysis or Product Specification Form.
Performance Data:
Resources:
Flyers:
Product Manual:
Technical Notes:
- MK-TN-0023 REV 01 Simplified RNA-Seq Library Prep Improved RNA-ASHG22
- MK-AN-0016_REV_01_Singular_Genomics_1222_lr
- Rapid 16S Metagenomic Library Preparation for Oxford Nanopore Technologies (ONT)® Platform
- sparQ UDI Adapter sequence overview
- Illumina Experiment Manager version 1.9 files (zip-download)
- Illumina Experiment Manager version 1.8 files (zip-download)
- Setting up sparQ Library Prep Kit in Illumina Experiment Manager
Brochures
CofA (PSFs)
SDSs