Reverse Transcriptase Selection Guide
Epicentre’s Reverse Transcriptases (see Table below) include MMLV High Performance RT, MonsterScript™ RT, and EpiScript™ RT. MMLV High Performance RT demonstrates significantly greater activity than other commercially available MMLV RT enzymes. MonsterScript RT has the ability to make longer cDNAs and more complete full-length libraries of first-strand cDNA molecules compared to other reverse transcriptases. EpiScript RT, an alternative to SuperScript® II RT, demonstrates more activity than other MMLV RT enzymes.
Enzyme | RNase H activity | Type | cDNA | Comparison | Optimal Temp | Heat Inactivation |
MMLV High Performance Reverse Transcriptase | Low | Recombinant | Full length (15 kb) | All MMLVs | 37-42°C | 85°C for 10 min |
MonsterScript™ Reverse Transcriptase | None | Recombinant | Full length (15 kb) | SuperScript® III | 50-60°C | 90°C for 5 min |
EpiScript™ Reverse Transcriptase | None detected | Recombinant | Full length (15 kb) | SuperScript® II | 37-42°C | 85°C for 5 min |