repliQa HiFi ToughMix
Superior speed and inhibitor tolerance
- High Fidelity – >90x wild type Taq
- Extreme Speed – up to 3x faster PCR results with extension rates as fast as 1 kb/sec*
- Tough Tested – tolerant to a wide range of PCR inhibitors
- Superior Sensitivity – higher yields from lower inputs as little as 2 pg
- Long Range – amplify +24 kb gDNA, +40 kb λDNA
*for fragments less than 1 kb in size
The repliQa HiFi ToughMix is a 2x, ready-to-use master mix that contains all the components for high fidelity PCR amplification, including a genetically modified DNA polymerase coupled with hot start antibodies. This unique, next generation master mix provides 90x higher fidelity compared to Taq, while reducing time to PCR results by 2-3x. The extreme speed is enabled by extension times as fast as 1-10 kb/sec depending on target length. The enzyme is coupled with the industry leading ToughMix which is tolerant to a wide variety of inhibitors making it suitable for routine PCR, cloning, amplicon sequencing and site directed mutagenesis.
repliQa HiFi ToughMix has fast extension times, ranging from 1-10 kb/sec depending on the fragment size, which significantly shortens time to results.
- Significantly faster results (up to 3x) with 1 kb/sec extension rate for fragments ≤1 kb
- No sacrifice in quality or yield of PCR results
repliQa HiFi ToughMix is able to tolerate a wide range of common PCR inhibitors, such as ethanol, hemoglobin and xylan, allowing for amplification of crude or difficult PCR sample types.
- Tolerant to a wide range of PCR inhibitors
- Amplify from crude samples
repliQa HiFi ToughMix provides higher yield and sensitivity, highlighting the enzyme’s high efficiency. Coupled with extreme amplification speed, PCR products are able to be detected earlier and at lower levels.
- Consistently achieve higher yields across a range of inputs
- Greater sensitivity with low inputs
repliQa HiFi ToughMix has the ability to amplify long fragments of genomic DNA (+24 kb) and lambda DNA (+40 kb).
- Amplify +24 kb gDNA and +40 kb lambda DNA
- Reduce time to result for longer fragments
- Amplify varying levels of GC-content targets (32%-70%)
repliQa HiFi ToughMix is able to amplify varying levels of GC-content targets (32%-70% GC-rich), further enabling superior PCR performance with bacterial, plant and mammalian sample types.
Contents:
2x reaction buffer containing optimized concentrations of MgCl2, dNTP’s and proprietarily formulated HiFi polymerase, hot start antibodies and ToughMix chemistry
Storage & Handling:
Store kit components in a constant temperature freezer at -25°C to -15°C upon receipt.
For lot specific expiry date, refer to package label, Certificate of Analysis or Product Specification Form.
Resources:
repliQa HiFi ToughMix is intended for molecular biology applications. This product is not intended for the diagnosis, prevention or treatment of a disease.
Ordering:
- 95200-025 repliQa HiFi ToughMix 25 Rxns
- 95200-100 repliQa HiFi ToughMix 100 Rxns
- 95200-500 repliQa HiFi ToughMix 500 Rxns