qScript XLT One-Step RT-qPCR ToughMix
Tough-tested 1-step real time PCR
![](/wp-content/uploads/2014/06/_d_improd_/95132_001_f_improf_434x289.jpg)
Features & Benefits
- Tough Tested – Overcomes common inhibitors including polysaccharides, heme/hemoglobin, humic acid, melanin
- Flexible – Use fast or standard qPCR cycling conditions
- Broad dynamic range- Reliable data from your precious samples every time
- Multiplexing enabled – Supports highly sensitive detection for up to four targets
qScript XLT 1-Step RT-qPCR ToughMix is intended for molecular biology applications. This product is not intended for the diagnosis, prevention or treatment of a disease.
qScript XLT One-Step RT-qPCR ToughMix is a ready-to-use, highly sensitive master mix for reverse transcription quantitative PCR (RT-qPCR) of RNA templates using hybridization probe detection chemistries such as TaqMan® 5′-hydrolysis probes. First-strand cDNA synthesis and subsequent PCR amplification are carried out seamlessly in the same reaction mixture with optimized 1-step thermal cycling parameters.
This kit is ideal for highly sensitive quantification of RNA viruses or low abundance RNA targets as well as high throughput gene-expression studies. The system has been optimized to deliver maximum RT-PCR efficiency, sensitivity, and specificity in minimal reaction volumes and accelerated thermal cycling rates. The only necessary user-supplied materials for RT-qPCR is RNA sample and probe assay. It is compatible with all types of molecular probe assays including dual-labeling strategies.
Elevating cDNA synthesis reaction temperature to 50-55°C during one-step RT-qPCR improves primer annealing and disruption of RNA secondary structure that can interfere with cDNA synthesis.
Contents
2X concentrated One-Step Master Mix containing:
- Reaction buffer with optimized concentrations of molecular-grade MgCl2, dATP, dCTP, dGTP, dTTP
- qScript XLT reverse transcriptase
- RNase inhibitor protein
- AccuStart II Taq DNA Polymerase
- Inert AccuVue™ plate loading dye
- Proprietary enzyme stabilizers and performance-enhancing additives
- Titrated reference dye (if applicable)
Storage & Handling
- Store components in a constant temperature freezer at -25°C to -15°C protected from light upon receipt.
- Repeated freezing and thawing does not affect RT-qPCR performance.
- For lot specific expiry date, refer to package label, Certificate of Analysis or Product Specification Form.
Resources
Customer Profile Stories
Flyers
Product Manuals
Application Notes
CofA (PSFs)
SDSs
- SDS 95132-02K qScript XLT 1-Step RT-qPCR ToughMix Kit, 2000R
- SDS 95132-05K qScript XLT One-Step RT-qPCR ToughMix 5000R
- SDS 95132-100 qScript XLT 1-Step RT-qPCR ToughMix Kit, 100R
- SDS 95132-500 qScript XLT 1-Step RT-qPCR ToughMix Kit, 500R
- SDS 95133-02K qScript XLT 1-Step RT-qPCR ToughMix ROX Kit, 2000R
- SDS 95133-100 qScript XLT 1-Step RT-qPCR ToughMix ROX Kit, 100R
- SDS 95133-500 qScript XLT 1-Step RT-qPCR ToughMix ROX Kit, 500R
- SDS 95134-02K qScript XLT 1-Step RT-qPCR ToughMix L-ROX Kit, 2000R
- SDS 95134-05K qScript XLT One-Step RT-qPCR ToughMix Low ROX
- SDS 95134-100 qScript XLT 1-Step RT-qPCR ToughMix L-ROX Kit, 100R
- SDS 95134-500 qScript XLT 1-Step RT-qPCR ToughMix L-ROX Kit, 500R
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