3rd July 2024

PerfeCTa SYBR Green SuperMix, UNG, Low ROX

PerfeCTa® SYBR® Green SuperMix, UNG, Low ROX™ is a 2X concentrated, ready-to-use reaction cocktail that contains all components, except primers and template for real-time quantitative PCR on Applied Biosystems 7500 or Stratagene MX series of real-time PCR systems. The proprietary buffer and stabilizers have been optimized exclusively for SYBR® Green I qPCR to deliver maximum PCR efficiency, sensitivity, and robust fluorescent signal. Highly specific amplification is crucial to successful qPCR with SYBR® Green I technology because this dye binds to and detects any dsDNA generated during amplification. A key component of this supermix is AccuStart™ Taq DNA polymerase which contains monoclonal antibodies that bind to the polymerase and keep it inactive prior to the initial PCR denaturation step. Upon heat activation (2 minutes at 95°C), the antibodies denature irreversibly, releasing fully active, unmodified Taq DNA polymerase. This enables specific and efficient primer extension with the convenience of room temperature reaction assembly. Additionally, the dNTP mix in this SuperMix contains dUTP in place of dTTP. Inclusion of uracil-N-glycosylase (UNG) prevents amplification of carry-over contamination from previous dU-containing PCRs.

Features & Benefits

  • High yield, high sensitivity
  • Highly specific amplification
  • Improved reproducibility and assay precision

Performance Data

Comparison to QuantiTect, GAPD

PageW - Graph Quanta vs Qiagen SYBR** - Quanta vs Qiagen SYBR

PerfeCTa SYBR Green SuperMix Comparison to QuantiTect SYBR Green PCR Kit (Qiagen), GAPD
Log-fold serial dilutions of qScript™ cDNA from HeLa cell total RNA (100 ng to 0.1 pg) were analyzed by SYBR Green qPCR following each manufacturers protocol. Averaged plots of triplicate qPCR reactions are shown.

Real-Time PCR Instrument Compatibility

  • Applied Biosystems 7500
  • Applied Biosystems 7500 Fast
  • Stratagene Mx3000P®
  • Stratagene Mx3005P™
  • Stratagene Mx4000™
  • Applied Biosystems ViiA 7
  • Applied Biosystems QuantStudio™ 12K Flex

Contents

  • 2X reaction buffer containing optimized concentrations of MgCl2, dNTPs (dATP, dCTP, dGTP, dUTP), AccuStart Taq DNA Polymerase, UNG, SYBR Green I dye, ROX Reference Dye (for 580-585 nm excitation) and stabilizers.

Storage conditions

PerfeCTa® SYBR Green SuperMix, UNG, Low ROX is stable for 1 year when stored in a constant temperature freezer at -20°C, protected from light. For convenience, it may be stored unfrozen at +2 to +8°C for up to 6 months. Repeated freezing and thawing of the supermix is not recommended. However, the product demonstrated no loss of performance after 20 freeze-thaw cycles or 2 months at +20°C.

Resources

Ordering

Cat# 95070-100 100 x 50-µl rxns (2 x 1.25 ml)
Cat# 95070-500 500 x 50-µl rxns (10 x 1.25 ml)
Cat# 95070-02K 2000 x 50-µl rxns (1 x 50 ml)