PerfeCTa qPCR SuperMix, UNG
PerfeCTa® qPCR SuperMix, UNG is a 2X concentrated, ready-to-use reaction cocktail that contains all components, except primers, probe(s), and template for real-time quantitative PCR systems that do not require an internal reference dye. The proprietary buffer and stabilizers have been specifically optimized to deliver maximum PCR efficiency, sensitivity, and robust fluorescent signal with TaqMan® or TaqMan MGB probe chemistry. The enhanced specificity of this supermix suppresses cross-reactivity between homologous sequences, improving detection and discrimination in SNP applications. A key component of this supermix is AccuStart™ Taq DNA polymerase which contains monoclonal antibodies that bind to the polymerase and keep it inactive prior to the initial PCR denaturation step. Upon heat activation (2 minutes at 95°C), the antibodies denature irreversibly, releasing fully active, unmodified Taq DNA polymerase. This enables specific and efficient primer extension with the convenience of room temperature reaction assembly. Additionally, the dNTP mix in this SuperMix contains dUTP in place of dTTP. Inclusion of uracil-N-glycosylase (UNG) prevents amplification of carry-over contamination from previous dU-containing PCRs.
Features & Benefits
- High yield, high sensitivity
- Highly specific amplification
- Superior reproducibility and precision
Real-Time PCR Instrument Compatibility
- Roche LightCycler™ 480
- Qiagen/Corbett Rotor-Gene® Q
- Qiagen/Corbett Rotor-Gene® 6000
- Qiagen/Corbett Rotor-Gene® 3000
- Eppendorf Mastercycler® ep realplex
- Eppendorf Mastercycler® ep realplex2 s
- Cepheid SmartCycler®
- Bio-Rad CFX96™
- Bio-Rad CFX384™
- Bio-Rad/MJ Opticon®
- Bio-Rad/MJ Opticon 2
- Bio-Rad/MJ Chromo4™
- Bio-Rad MiniOpticon™
- Illumina Eco qPCR
- Thermo Scientific PikoReal Cycler
- BioRad iCycler iQ™
- BioRad MyiQ™
- BioRad iQ™5
Contents
- 2X reaction buffer containing optimized concentrations of MgCl2, dNTPs (dATP, dCTP, dGTP, dUTP), AccuStart Taq DNA Polymerase, UNG, and stabilizers.
Storage conditions
PerfeCTa® qPCR SuperMix, UNG is stable for 1 year when stored in a constant temperature freezer at -20°C. For convenience, it may be stored unfrozen at +2 to +8°C for up to 6 months. Repeated freezing and thawing of the supermix is not recommended. However, the product demonstrated no loss of performance after 20 freeze-thaw cycles or 2 months at +20°C.
Resources
Ordering
| Cat# 95064-100 | 100 x 50µl rxns (2 x 1.25 ml) | |
| Cat# 95064-500 | 500 x 50µl rxns (10 x 1.25 ml) | |
| Cat# 95064-02K | 2000 x 50µl rxns (1 x 50 ml) |