PerfeCTa qPCR FastMix, UNG
PerfeCTa® qPCR FastMix™ is a 2X concentrated, ready-to-use reaction cocktail containing all components except primers, probe and template for real-time quantitative PCR systems that do not require an internal reference dye. Quanta FastMixes™ include buffers and stabilizers optimized for maximum PCR efficiency, reproducibility and sensitivity with TaqMan® and TaqMan® MGB probe chemistries. A key component of PerfeCTa® qPCR FastMix is our AccuFast™ Taq DNA polymerase that enables instant activation and rapid polymerization kinetics. Upon heat activation (20 seconds at 95°C), the antibodies irreversiblydenature releasing fully active, unmodified Taq DNA polymerase. This promotes specific and efficient primer extension with the convenience of room temperature reaction assembly. PerfeCTa® qPCR FastMix™ delivers maximum PCR efficiency, sensitivity, specificity, and robust fluorescent signal with either Fast or conventional cycling conditions.
Features & Benefits
- Shorter run times – enabling more experiments per day
- Broad dynamic range – more reliably attain useful data from your precious samples
- Superior antibody-mediated hot-start – higher specificity leading to more accurate quantification
- Pre-optimized formulation – multiplexing, fast cycling or UNG formats save time
Real-Time PCR Instrument Compatibility
- Roche LightCycler™ 480
- Qiagen/Corbett Rotor-Gene® Q
- Qiagen/Corbett Rotor-Gene® 6000
- Qiagen/Corbett Rotor-Gene® 3000
- Eppendorf Mastercycler® ep realplex
- Eppendorf Mastercycler® ep realplex2 s
- Cepheid SmartCycler®
- Bio-Rad CFX96™
- Bio-Rad CFX384™
- Bio-Rad/MJ Opticon®
- Bio-Rad/MJ Opticon 2
- Bio-Rad/MJ Chromo4™
- Bio-Rad MiniOpticon™
- Illumina Eco qPCR
- Thermo Scientific PikoReal Cycler
- BioRad iCycler iQ™
- BioRad MyiQ™
- BioRad iQ™5
Contents
- 2X reaction buffer containing optimized concentrations of MgCl2, dNTPs (dATP, dCTP, dGTP, dTTP), AccuFast Taq DNA Polymerase, UNG, and stabilizers
Storage conditions
PerfeCTa™ qPCR FastMix™, ROX is stable for 1 year when stored in a constant temperature freezer at -20°C. For convenience, it may be stored unfrozen at +2 to +8°C for up to 6 months. After thawing, mix thoroughly before using. Repeated freezing and thawing of the supermix is not recommended. However, the product demonstrated no loss of performance after 20 freeze-thaw cycles or 2 months at +20°C.
Resources
Ordering
| Cat# 95076-250 | 250 x 20-µl rxns (2 x 1.25 ml) | |
| Cat# 95076-012 | 1250 x 20-µl rxns (10 x 1.25 ml) | |
| Cat# 95076-05K | 5000 x 20-µl rxns (1 x 50 ml) |