PerfeCTa MultiPlex qPCR ToughMix, Low ROX

PerfeCTa MultiPlex qPCR ToughMix Low ROX is a 5X concentrated, ready-to-use reaction cocktail for real-time quantitative PCR (qPCR) that overcomes many known inhibitors of PCR often present in crude samples extracted from environmental specimens, plant tissues, or animal tissues. It contains all components, except primers, probes and templates. The 5X concentrated ToughMix allows addition of higher amounts of template and improved detection sensitivity with low concentration samples. PerfeCTa MultiPlex qPCR ToughMix Low ROX has been optimized to deliver maximum PCR efficiency, sensitivity, and specificity in reduced reaction volumes with fast cycle or conventional PCR cycling protocols.The system transcends multiplex limitations of conventional PCR master mixes, enabling unbiased amplification of up to five target sequences in a single tube. Suppression of low copy amplicons by high copy reference targets in the amplification is a common problem in multiplex PCR that can skew, or mask the apparent representation and quantification of low copytarget sequences. PerfeCTa MultiPlex qPCR ToughMix results in multiplexed qPCR with dynamic range and sensitivity that are comparable to single-plex qPCR probe assays without the need for limiting or variable primer concentrations.A key component of PerfeCTa MultiPlex qPCR ToughMix Low ROX is an ultra pure, highly processive thermostable DNA polymerase that is combined with high avidity monoclonal antibodies. This proprietary polymerase mix is highly resistant to PCR inhibitors and provides an extremely stringent automatic hot-start allowing reaction assembly, and temporary storage, at room temperature prior to PCR amplification.

Performance Data

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4-Plex Bias vs. 1-Plex

Real-Time PCR Instrument Compatibility

• Applied Biosystems 7500
• Applied Biosystems 7500 Fast
• Stratagene Mx3000P®
• Stratagene Mx3005P™
• Stratagene Mx4000™
• Applied Biosystems ViiA 7
• Applied Biosystems QuantStudio™ 12K Flex

Contents

• PerfeCTa Multiplex qPCR ToughMix Low ROX 5X reaction buffer containing optimized concentrations of MgCl2, dNTPs (dATP, dCTP, dGTP, dTTP), hot-start DNA polymerase, ROX reference dye and stabilizers.

Storage conditions

PerfeCTa Multiplex qPCR ToughMix Low ROX is stable for 2 years when stored in a constant temperature freezer at -20°C, protected from light. For convenience, it may be stored unfrozen at +2 to +8°C for up to 6 months. Repeated freezing and thawing of the ToughMix will not affect product performance.

Precautions and Disclaimer

For research use only. Not intended for any animal or human therapeutic or diagnostic use.
Please consult the Material Safety Data Sheet for information regarding hazards and safe handling practices.

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