PerfeCTa® MultiPlex qPCR SuperMix, Low ROX™

PerfeCTa Multiplex qPCR SuperMix, Low ROX is a 2X concentrated, ready-to-use reaction cocktail that contains all components, except primers, probe(s), and template for real-time quantitative PCR on Applied Biosystems 7500 or Stratagene MX series of real-time PCR systems. The system transcends multiplex limitations of conventional PCR master mixes, enabling unbiased amplification of up to five target sequences in a single tube. Suppression of low copy amplicons by high copy reference targets in the amplification is a common problem in multiplex PCR. This can skew, or mask the apparent representation and quantification of low copy target sequences. PerfeCTa Multiplex qPCR SuperMix, Low ROX delivers dynamic range and sensitivity to multiplexed qPCR that is comparable to that for singleplex qPCR probe assays without the need for limiting or variable primer concentrations. A key component of this supermix is AccuStart™ Taq DNA polymerase which contains monoclonal antibodies that bind to the polymerase and keep it inactive prior to the initial PCR denaturation step. Upon heat activation (2 minutes at 95°C), the antibodies denature irreversibly, releasing fully active, unmodified Taq DNA polymerase. This enables specific and efficient primer extension with the convenience of room temperature reaction assembly.

Real-Time PCR Instrument Compatibility

• Applied Biosystems 7500
• Applied Biosystems 7500 Fast
• Stratagene Mx3000P®
• Stratagene Mx3005P™
• Stratagene Mx4000™
• Applied Biosystems ViiA 7
• Applied Biosystems QuantStudio™ 12K Flex

Contents

• 2X reaction buffer containing optimized concentrations of MgCl2, dNTPs (dATP, dCTP, dGTP, dTTP) AccuStart Taq DNA Polymerase, ROX Reference Dye (for 580-585 nm excitation), and stabilizers.

Storage conditions

PerfeCTa Multiplex, Low ROX qPCR SuperMix is stable for 1 year when stored in a constant temperature freezer at -20°C, protected from light. For convenience, it may be stored unfrozen at +2 to +8°C for up to 6 months. Repeated freezing and thawing of the supermix is not recommended.

Precautions and Disclaimer

For research use only. Not intended for any animal or human therapeutic or diagnostic use. Please consult the Material Safety Data Sheet for information regarding hazards and safe handling practices.

Ordering

Cat# 95108-050	50 x 50-µl rxns (1 x 1.25 ml)
Cat# 95108-200	200 x 50-µl rxns (4 x 1.25 ml)
Cat# 95108-01K	1000 x 50-µl rxns (1 x 25 ml)