Biotium One Step Blue Protein Gel Stain
Using Coomassie Blue? Biotium One-Step Blue Protein Gel Stain is a better solution!
One Step Blue Protein Gel Stain is a ready-to-use protein gel staining solution. It produces fast (5-60 min) protein staining in a single step without fixation or washing. Proteins can be detected by visible blue staining, or by near-infrared fluorescence. In addition to rapid results and simple staining, One-Step Blue™ offers safer handling and disposal compared to Coomassie staining because it is entirely aqueous-based, without hazardous methanol or acetic acid.
The staining is fully compatible with mass spectrometry and Edman-based sequencing.
One-step staining, no fixing, no washing.
100% water based formulation.
Replaces tedious Coomassie staining for visible blue imaging of bands.
One-Step Blue™ has comparable sensitivity as the widely used Coomassie Blue, with a lower limit of detection around 10-20 ng depending on the detection method used. Note that staining intensity varies between proteins.
Bands visible after 5 minutes, maximum sensitivity after 60 minutes, destain optional.
Optional near-infrared fluorescence detection with NIR imaging systems for higher sensitivity and convenient quantitation.
Dispose down the drain after neutralization.
Biotium One Step Blue Protein Gel Stain is available in 1L and 4L sizes.
Cat # 21003-1L: One Step Blue Protein Gel Stain 1L
Cat # 21003-4L: One Step Blue Protein Gel Stain 4L