GenepHlow™ DNA Cleanup Midi Kit (DFI100, DFI300)
GenepHlow™ DNA Cleanup Midi Kits were designed to recover or concentrate DNA fragments from PCR or other enzymatic reactions. This DNA cleanup kit includes a pH indicator as an optional addition to the PB binding buffer to ensure optimal pH and facilitate DNA binding. If pH exceeds the optimal level (>7.5), the color of the solution will appear purple instead of yellow. 3M Sodium Acetate (pH5.0) which is included with the kit, can then be added to the solution to adjust pH and return the color to yellow. Chaotropic salt is used to denature enzymes while DNA fragments are bound by the glass fiber matrix of the spin column. Contaminants are removed with a Wash Buffer (containing ethanol) and the purified DNA fragments are eluted by a low salt Elution Buffer, TE or water. The pH indicator, salts, enzymes and unincorporated nucleotides can be effectively removed from the reaction mixture without phenol extraction or alcohol precipitation and the purified DNA is ready for use in subsequent reactions.
Advantages
- High Efficiency: up to 90% recovery
- Convenient: includes pH indicator for easy determination of optimal pH and sodium acetate to adjust pH if it becomes too high
- Includes 3M Sodium Acetate (pH5.0) to adjust pH if it becomes too high
- Sample Size: 10-40 µg of DNA in up to 200 µl PCR/Digestion solutions
- Broad Fragment Size Range: 100 bp–20 kb
- Operation Time: 10 minutes
- Spin Columns: glass fiber membrane
- Elution Volume: 30-50 µl
- Storage: dry at room temperature (15-25°C)
Applications
Fluorescent and Radioactive Sequencing, PCR, Restriction Enzyme Digestion, DNA Labeling and Ligation, Microarray, Transfection
Components
- PB Buffer
- pH Indicator
- 3M Sodium Acetate (pH5.0)
- Wash Bufffer
- Elution Buffer (10 mM Tris-HCl, pH8.5 at 25ºC)
- DF Midi Columns
- 2 ml Collection Tubes
Quality Control
The quality of the GenepHlow™ DNA Cleanup Midi Kit is tested on a lot-to-lot basis by purifying DNA fragments of various sizes from PCR products, digestion products or other aqeous solutions. The purified DNA is analyzed by electrophoresis.
Ordering:
- Cat # DFI100, DFI300)
Resources: