Biotium DNAzure™ Blue Nucleic Acid Gel Stain, 100X
DNAzure™ Blue Nucleic Acid Gel Stain, 100X is an ultrasensitive reagent for visible staining of dsDNA in agarose gels or polyacrylamide gels.
Visualize DNA bands in gels by eye, with detection sensitivity rivaling most fluorescence-based gel stains.
Key to the technology is a DNA-binding dye that turns from colorless to deep blue upon exposure to light.
The benefits include:
- Deep blue bands visible by the naked eye following 5-30 min light exposure
- Ultrasensitive detection, as little as ~1 ng DNA
- Simplified DNA band excision, without the need for DNA damaging UV light
- Compatible with downstream applications such as sequencing and cloning
- Expensive gel documentation systems not required for imaging
Biotium’s 1 kb DNA ladder was loaded on a 1% agarose gel in two-fold dilutions, ranging from 200 ng to 25 ng total ladder per lane. The mass of the 500 bp band in each lane is labeled. The gel was stained with DNAzure™ Blue Nucleic Acid Gel Stain for 25 minutes, and then the visible blue DNA bands were developed for 30 minutes using the Glo=Plate™ Blue LED transilluminator. The gel was placed on a white light transilluminator and imaged with a cell phone camera.
After incubation with DNAzure Blue Nucleic Acid Gel Stain, the gel must be exposed to bright light to facilitate the development of dark blue DNA bands.
Light exposure can be performed with a variety of white and blue light sources. For best results, we recommend performing the light exposure with either the Gel-Bright™ LED Gel Illuminator or the Glo-Plate™ Blue LED Illuminator. Both devices produce blue light from LEDs and are very effective at rapidly producing the color change in DNAzure™-stained gels. The Gel-Bright™ is also an excellent instrument for viewing gels stained with the fluorescent green DNA gel stain, GelGreen™.
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