AccuStart Long Range SuperMix
Superior sensitivity and multiplexing for DNA amplification of long targets.
Features & Benefits:
- Amplify +24 kb gDNA and +40 kb lambda DNA
- 4x concentration enables superior sensitivity with low inputs (100 pg)
- Multiplex up to 6 targets with each target up to 6 kb in length
- Stabilized, single-tube SuperMix minimizes pipetting errors and hands-on-time
- Reliable lot-to-lot reproducibility ensures consistent resultsAccuStart Long Range SuperMix is intended for molecular biology applications. This product is not intended for the diagnosis, prevention or treatment of a disease.
The AccuStart Long Range SuperMix is a 4x, ready-to-use solution that contains all the components for long range target amplifications, including a blend of two hot-start thermostable DNA polymerases (one with strong proof-reading activity) and an optimized buffer to ensure high efficiency, sensitivity and specificity.
The SuperMix enables routine and easy amplification of up to 24kb target from gDNA with high accuracy (> 10-fold better fidelity than Taq) and accommodates targets with broad GC-content (no separate GC buffer needed). This product is also capable of amplifying multiple targets simultaneously and is suitable for end point PCR, template prep for sanger sequencing, NGS, cloning and HLA typing.
A: Comparison of sensitivity and yield. An 8.5 kb β-globin was amplified in 50 µL reaction volumes according to the recommended protocol. Reaction inputs varied from 100 pg – 10 ng. Following a 3 min activation at 95°C; 30 cycles of: 92°C, 30 s; 65°C, 6 min; 72°C, 10 min. 5 µL of PCR products were analyzed on a 0.5% agarose gel with a DNA marker.
B: Comparison of yield over fragment length. 8.5 kb β-globin and 24 kb tPA fragments were amplified in 50 μl reaction volumes according to the recommended protocol. Reactions inputs were 5 ng and 25 ng for the 8 kb and 24 kb fragments, espectively. Following a 3 min activation at 95°C; 27 cycles of: 92°C, 30 s; 65°C, 6 min (8.5 kb), 12 min (24 kb); 72°C, 10 min. 5 μl of PCR products were analyzed on a 0.5% agarose gel with a DNA marker.
- Higher yield than major competitors
- Inputs as low as 100 pg
- Strong yield at 24 kb length
- Clean and visible PCR bands
Strong Multiplexing Capabilities Multiplex PCR reactions were performed for amplification of 2kb, 3kb, 4kb and 5kb BRCA 1 targets with 1kb and 6kb BRCA 2 targets from 10 ng human genomic DNA template. Reactions were run on MJ Research PTC-200 Thermal Cycler. Following a 2 min activation of 95°C; 27 cycles of: 92°C, 30 s, 65°C, 8 min); 72°C, 10 min. 4 µL of PCR products were analysed on a 0.7% agarose gel.
- Multiplex 6 targets up to 6 kb each
- Reduction of costs and increase in the speed of experiments
- More data generated per run
Lot-to-Lot Consistency8.5 kb β-globin, 17.5 kb and 24 kb tPA fragments were amplified in 50 µL reaction volumes according to the recommended protocol. Reactions inputs were 5 ng, 10 ng and 25 ng for the 8 kb, 17.5 kb and 24 kb fragments, respectively. Following a 3 min activation at 95°C; 27 cycles of: 92°C, 30 s; 65°C, 6 min (8.5 kb), 8 min (17.5 kb), 12 min (24 kb); 72°C, 10 min. 5 µL of PCR products were analyzed on a 0.5% agarose gel with a DNA.
- Strong consistency across three separate lots
- Manufactured in state of the art ISO 13485 facility
Details:
- Contents
- 4x ready-to-use solution
- Storage & Handling
- Store kit components in a constant temperature freezer at -25°C to -15°C upon receipt. For lot specific expiry date, refer to package label, Certificate of Analysis or Product Specification Form.
Store kit components in a constant temperature freezer at -25°C to -15°C upon receipt. For lot specific expiry date, refer to package label, Certificate of Analysis or Product Specification Form.