AccuClear Ultra High Sensitivity dsDNA Quantitation Kit with 1 DNA Standard
AccuClear™ Ultra High Sensitivity dsDNA Quantitation Solution utilizes a novel, next-generation DNA binding dye for highly sensitive and accurate quantitation of purified dsDNA samples across a broad range of DNA concentrations. The assay is linear between 0.03 ng and 250 ng of dsDNA per assay in microplate format. Unlike absorbance-based measurements, AccuClear™ dye is highly selective for double-stranded DNA over single stranded DNA or RNA.
The AccuClear™ dsDNA quantitation assay is designed for use with fluorescence 96-well plate readers equipped with excitation and emission filters for detecting green fluorescence. The unique spectral properties of AccuClear™ dye make it especially well-suited for use with instruments with blue LED excitation sources. Biotium’s AccuLite™ 470 Mini Fluorometer (cat. no. E90001) includes a program for use with the AccuClear™ assay. AccuClear™ also is compatible with handheld fluorometers such as Invitrogen’s Qubit® and Promega’s QuantiFluor®-P, however the standard curve calibration programs for these instruments may not cover the full dynamic range of the AccuClear™ kit standard curve.
The AccuClear™ Ultra High Sensitivity dsDNA Quantiation Kit with 1 DNA Standard contains AccuClear™ dye, 20X assay buffer, and a 25 ng/uL calf thymus dsDNA standard stock solution that can be used to prepare a set of standards. Biotium also offers the AccuClear™ Ultra High Sensitivity dsDNA Quantiation Kit with 7 DNA Standards (1000 assays, catalog no. 31028), which includes AccuClear™ dye, 1X assay buffer, and a set of prediluted calf thymus dsDNA standards. AccuClear™ Ultra High Sensitivity dsDNA Quantitation Solution (1000 assays, catalog no. 31027), which includes dye and 1X buffer without standards, is available for customers who wish to prepare their own DNA standards.
Standard curve of calf thymus DNA assayed using the AccuClear Ultra High Sensitivity Kit and read on a microplate reader (Ex/Em 460/507).
Inset shows the lower end of the titration.
Components
- AccuClear™ Dye (100X)
- AccuClear™ Buffer
- Calf Thymus dsDNA Standard, 25 ng/uL
Features
- Linear range: 0.03-250 ng dsDNA
- Green fluorescence (Ex/Em 460/507 nm)
- Unrivaled sensitivity and dynamic range
- Novel green fluorescent dye is a perfect match for blue LED excitation sources
- Green fluorescence detection compatible with fluorescence microplate reader, handheld fluorometer, or NanoDrop® fluorospectrophotometer.
Please also see related products for information on the AccuBlue™ High Sensitivity dsDNA Quantitation Solution and Kit (0.2-100 ng) and AccuBlue™ Broad Range dsDNA Quantiation Solution and Kit (2-2000 ng), In addition to different linear ranges, these assays have different tolerances to various contaminants compared to AccuClear™ (see product protocols for details).
AccuClear kits are intended for quantitation of purified dsDNA samples.
AccuClear™ technology is covered by pending U.S. and international patents.
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Ordering
Cat # 31029 – 2,000 assays
