AccuBlue High Sensitivity dsDNA Quantitation Solution

AccuBlue™ High Sensitivity dsDNA Quantitation Solution offers ultra-sensitive and selective detection of dsDNA with minimal effects from common contaminants. The assay provides a linear detection range from 0.2 – 100 ng with sufficient reagents to perform 1000 assays based on a 96-well microplate format.

AccuBlue™ High Sensitivity Quantitation Solution also is available with pre-diluted DNA standards (Catalog #31006). The AccuBlue™ High Sensitivity assay is superior to PicoGreen® or Quant-iT® dsDNA detection kits by having a similar detection range but with more convenience and better safety. The high sensitivity reagent is impermeable to cell membranes, whereas PicoGreen® or the high sensitivity Quant-iT® DNA-binding dye readily crosses cell membranes. Moreover, the reagent is extremely stable.

AccuBlue™ can be conveniently stored at 4°C, unlike PicoGreen® or Quant-iT® reagents, which must be aliquoted for storage to avoid multiple freeze-thaw cycles. Biotium’s AccuLite™ 470 Mini Fluorometer includes a program for use with the AccuBlue™ High Sensitivity assay. The assay (λ_Ex/λ_Em: 485/530 nm) also is compatible with microplate readers, spectrofluorometers and hand-held minifluorometers such as the Qubit® from Invitrogen. Please also see AccuBlue™ Broad Range dsDNA Quantitation Kit for quantitation of 2-2000 ng and AccuClear™ Ultra High Sensitivity dsDNA Quantitation Kit for quantitation of 0.03-250 ng DNA.

Standard curve of calf thymus DNA assayed using the AccuBlue High Sensitivity Kit and read on a microplate reader (Ex/Em 485/530). Inset shows the lower end of the titration.

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Cat# 31008 – 1,000 assays
Cat # 31008-T – 200 assays (trial size)