AccuBlue High Sensitivity dsDNA Quantitation Kit with 8 DNA Standards
The AccuBlue™ High Sensitivity dsDNA Quantitation Kit offers ultra-sensitive and selective detection of purified dsDNA samples with minimal effects from common contaminants. The assay provides a linear detection range from 0.2 – 100 ng with sufficient reagents to perform 1000 assays based on a 96-well microplate format. The 1000 assay size kit includes AccuBlue™ High Sensitivity Quantitation Solution components and a set of 8 pre-diluted calf thymus dsDNA standards. The trial size kit includes one DNA standard solution with instructions for preparing a set of standards by serial dilution. AccuBlue™ High Sensitivity Quantitation Solution also is available separately (Catalog #31008) for customers who wish to use their own DNA standards.
The AccuBlue™ High Sensitivity assay is superior to PicoGreen® or Quant-iT® dsDNA detection kits by having a similar detection range but with more convenience and better safety. The high sensitivity reagent is impermeable to cell membranes, whereas PicoGreen® or the high sensitivity Quant-iT® DNA-binding dye readily crosses cell membranes. Moreover, the reagent is extremely stable. AccuBlue™ can be conveniently stored at 4°C, unlike PicoGreen® or Quant-iT® reagents, which must be aliquotted for storage to avoid multiple freeze-thaw cycles.
Standard curve of calf thymus DNA assayed using the AccuBlue High Sensitivity Kit and read on a microplate reader (Ex/Em 485/530).
Inset shows the lower end of the titration.
Biotium’s AccuLite 470 Mini Fluorometer includes a program for use with the AccuBlue™ High Sensitivity assay. The assay (λEx/λEm 485/530 nm) also is compatible with microplate readers, spectrofluorometers and hand-held minifluorometers such as the Qubit® from Invitrogen.
Please also see AccuBlue™ Broad Range dsDNA Quantitation Kit for quantitation of 2-2000 ng and AccuClear™ Ultra High Sensitivity dsDNA Quantitation Kit for quantitation of 0.03-250 ng DNA.
AccuBlue™ technology is covered by U.S. Patent No. 8,148,515.
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Cat# 31006 – 1,000 assays
