Accel-Amplicon™ Plus EGFR Pathway Panel
All-in-One Amplicon Solution
The Accel-Amplicon™ EGFR Pathway Panel offers contiguous coverage of EGFR and hotspot coverage of BRAF, KRAS, and NRAS, using a 17-amplicon design to generate multiplex libraries compatible with Illumina sequencing platforms. The Accel-Amplicon EGFR Pathway Panel is compatible with short DNA fragments from both FFPE and cfDNA samples.
This is especially well-suited for research focused on detecting clinically-relevant allele variants in DNA from circulating tumor cells (CTCs). This product is a complete kit that includes all elements necessary for generating ready-to-sequence libraries, including primer pairs and indexed sequencing adapters.
Features:
- Single-tube assay
- Ready-to-sequence libraries in 2 hours
- Inputs as low as 10 ng
Benefits:
- Average amplicon size of 136 bp for compatibility with cfDNA and FFPE
- Limit of detection as low as 1%
- On-target specificity and coverage uniformity > 95%
- Leverages the high fidelity performance of the Illumina platform
- Complete library generation in a single kit
Workflow:
The Accel-Amplicon workflow uses a fast, single-tube approach consisting of a 90-minute target enrichment amplification step and a 10-minute adapter ligation step, yielding a 2-hour start-to-finish procedure.
Product Specifications:
Decrease Input, Not Sensitivity
The Accel-Amplicon EGFR Pathway Panel offers high sensitivity variant detection from input amounts from 10-25 ng. The kit utilizes Illumina-compatible dual-indexed adapter sequences and has been validated on the MiSeq® and HiSeq® platforms.
Performance Data:
Resources:
Protocols & Tools
- Protocol – Accel Amplicon & Accel Amplicon Plus Panels
- Protocol – Input DNA Quantification Assay
- Accel-Amplicon Master Mixing Volume Calculator
Custom Target Submission Form
Ordering:
Both catalog numbers, ordered as is, provide the same panel content; however, you may add additional targets to AP-EG8048 to customize your assay. Please use the Custom Target Submission Form to add additional content.
- Cat # AP-EG8048 Accel-Amplicon Plus EGFR Pathway Panel (48 rxns)
- Cat #AL-51048 Accel-Amplicon EGFR Pathway Panel (48 rxns)
The below solution is included with your product. If you require extra, please advise: - Cat # 90196 PEG NaCl Solution (96 rxns)