DNA & RNA Quantification via Fluorometric Analysis

Nucleic acid quantification, encompassing double stranded DNA (dsDNA), single stranded DNA (ssDNA) and RNA quantification, is a staple process in life science applications requiring knowledge of sample concentration  prior to downstream experiments. Both types of nucleic acid are typically quantified using one of two optical technologies: ultraviolet-visible (UV-Vis) spectroscopy, or …

Background Reducers for Improved Fluorescent Stains

Christopher Pratt, PhD October 9, 2020. Like a bright sky obscuring the stars, background fluorescence in microscopy makes specific signals harder to see—only the brightest points shine through. Reducing background improves signal-to-noise and enhances image quality, producing better data. Both the intrinsic fluorescence of samples (autofluorescence) and non-specific binding of …