Biotium 100 bp DNA Ladder

100 bp DNA Ladder is suitable for sizing linear double-stranded DNA fragments from 100 bp to 1500 bp. The ladder consists of 11 bands that are generated from PCR and restriction enzyme digestion of double-stranded DNA. The DNA is purified by phenol extraction, and equilibrated to 10 mM Tris-HCl (pH 8.0) and 1 mM EDTA. The 500 bp and 1,500 bp bands have increased intensity to provide internal orientation.

The ladder is supplied at 100 ng/uL in 10 mM Tris pH 8.0, 1 mM EDTA, together with 1.5 mL of 6X Loading Buffer containing density agents and two blue electrophoresis tracking dyes that run at approximately 1.5 kb and 200 bp in a 1% agarose gel.

Biotium also offers Ready-to-Use 100 bp DNA Ladder, 1 kb DNA ladder, and Ready-to-Use 1 kb DNA ladder.

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