PerfeCTa® MultiPlex qPCR SuperMix
PerfeCTa® Multiplex qPCR SuperMix is a 2X concentrated, ready-to-use reaction cocktail for real-time quantitative PCR (qPCR) that contains all components, except primers, probes and templates. The system transcends multiplex limitations of conventional PCR master mixes, enabling unbiased amplification of up to five target sequences in a single tube. Suppression of low copy amplicons by high copy reference targets in the amplification is a common problem in multiplex PCR. This can skew, or mask the apparent representation and quantification of low copy target sequences. PerfeCTa® Multiplex qPCR SuperMix delivers dynamic range and sensitivity to multiplexed qPCR that is comparable to that for singleplex qPCR probe assays without the need for limiting or variable primer concentrations. A key component of this supermix is AccuStart™ Taq DNA polymerase which contains monoclonal antibodies that bind to the polymerase and keep it inactive prior to the initial PCR denaturation step. Upon heat activation (2 minutes at 95°C), the antibodies denature irreversibly, releasing fully active, unmodified Taq DNA polymerase. This enables specific and efficient primer extension with the convenience of room temperature reaction assembly.
Features & Benefits
- Real-time multiplex detection of up to 5 targets
- Linearity over 6 orders of magnitude of input cDNA and 4 orders of magnitude of input genomic DNA
- Detect up to 4 targets when one differs in expression up to 106-fold relative to the others
Contents
- PerfeCTa® Multiplex qPCR Supermix – 2X reaction buffer containing optimized concentrations of MgCl2, dNTPs (dATP, dCTP, dGTP, dTTP), AccuStart Taq DNA Polymerase, and stabilizers.
- ROX Reference Dye (50X) – 50X concentrated ROX solution optimized for Applied Biosystems 7000, 7300, 7700, 7900, or 7900HT
- Low ROX Reference Dye (50X) – 50X concentrated ROX solution optimized for Applied BioSystems 7500 or Stratagene MX4000™, MX3005P™, MX3000P™
Storage conditions
PerfeCTa® Multiplex qPCR SuperMix is stable for 1 year when stored in a constant temperature freezer at -20°C, protected from light. For convenience, it may be stored unfrozen at +2 to +8°C for up to 6 months. Repeated freezing and thawing of the supermix is not recommended.
Resources
Ordering
| Cat# 95063-050 | 50 x 50-µl rxns (1 x 1.25 ml) | ||
| Cat# 95063-200 | 200 x 50-µl rxns (4 x 1.25 ml) | ||
| Cat# 95063-01K | 1000 x 50-µl rxns (1 x 25 ml) |