Terminator™ 5´-Phosphate-Dependent Exonuclease
Applications
Note: For RNA-Seq applications, we recommend using a Ribo-Zero™ Kit. Terminator™ 5´-Phosphate-Dependent Exonuclease* is a processive 5´→3´ exonuclease that digests RNA that has a 5´-monophosphate end. It does not digest RNA that has a 5´-triphosphate, 5´-cap or 5´-hydroxyl group. Terminator Exonuclease is not inhibited by proteinaceous RNase inhibitors, such as Epicentre’s ScriptGuard™ RNase Inhibitor or placental ribonuclease inhibitors. Unit Definition: One unit of Terminator™ Exonuclease digests 1 µg of rRNA substrate to acid-soluble nucleotides in 60 minutes at 30°C under standard assay conditions. Storage Buffer: 50% glycerol containing 0.05 M Tris-HCl (pH 7.5), 100 mM NaCl, 0.1 mM EDTA, 1 mM DTT, and 0.1% Triton™ X-100. |
 Figure 1. A 1-hour Terminator™ Exonuclease reaction digests the large rRNAs in a eukaryotic or prokaryotic total RNA sample, producing an enriched mRNA preparation. |
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| Figure 2. Normal rat kidney (NRK) total RNA before (A) and after (B) Terminator™ Exonuclease treatment. The Terminator Exonuclease-treated RNA was concentrated 10-fold. | Figure 3. Denaturing agarose gel analysis of E. coli total RNA before (-) and after (+) Terminator™ Exonuclease digestion. The Terminator Exonuclease-treated RNA was concentrated 10-fold. |
| Figure 4. (click to enlarge) Overview of a process successfully used at Epicentre to characterize the 5´-terminal end group of RNAs using Terminator™ 5´-Phosphate-Dependent Exonuclease (Terminator Exo), alkaline phosphatase (AP) and tobacco acid pyrophosphatase (TAP). RT-PCR was used to monitor the status of individual transcripts. |
*Patent pending.
Ordering
| Catalog No. | Concentration | Size |
|---|
Terminator™ 5´-Phosphate-Dependent Exonuclease
| TER51020 | 1 U/µl | 40 Units |
Includes 10X Terminator™ Reaction Buffers A and B.