AccuStart™ Taq DNA Polymerase
Description
AccuStart Taq DNA Polymerase is a recombinant Taq DNA polymerase preparation which contains monoclonal antibodies that bind to the polymerase and keep it inactive before PCR thermal cycling. Upon heat activation (1 minute at 94°C), the antibodies denature irreversibly, releasing fully active Taq DNA polymerase. Non-specific extension of primers at low temperatures is a common cause of artifacts and poor sensitivity in PCR. The AccuStart automatic hot-start enables specific and efficient primer extension in the PCR process with the added convenience of room temperature reaction assembly. Activated AccuStart Taq DNA polymerase possesses 5’→3′ DNA polymerase activity and a double-strand specific 5’→3′ exonuclease. The polymerase does not have 3′-exonuclease activity and is free of any contaminating endo or exonuclease activities. One unit is defined as the amount of enzyme that will incorporate 10 nmol of dNTP into acid-insoluble material in 30 minutes at 74°C.
Contents
- AccuStart Taq DNA polymerase – 5 units/µL in 50% glycerol, 20 mM Tris-HCl, 40 mM NaCl, 0.1 mM EDTA, and stabilizers.
- 10X PCR Buffer – 0.2M Tris-HCl (pH 8.4), 0.5 M KCl
- 50 mM magnesium chloride – 50 mM MgCl2
Storage conditions
AccuStart Taq DNA polymerase is stable for 2 years when stored in a constant temperature freezer at -20°C.
Resources
Ordering
Cat# 95061-250 250 units; 5 units/µl
Cat# 95061-01K 1000 units; 5 units/µl
Cat# 95061-05K 5000 units; 5 units/µl