Exo-Minus Klenow DNA Polymerase (D355A, E357A)
Note: This product replaces Exo-Minus Klenow DNA Polymerase (Cat Nos. KL0810250, KL081001K, KL105000, and KL080911K), which will be discontinued effective May 31, 2012.
Applications
- Random-primer labeling of DNA.1
- Second-strand cDNA synthesis.2
- Strand-displacement amplification.3
Exo-Minus Klenow DNA Polymerase is a DNA-dependent DNA polymerase that lacks both the 5´→3´ and 3´→5´ exonuclease activities of E. coli DNA Polymerase I, from which it is derived. This N-terminal truncation of DNA Polymerase I has two mutations (D355A and E357A).
Unit Definition: One unit of Exo-Minus Klenow DNA Polymerase (D355A, E357A) converts 10 nmol of dNTPs into acid-insoluble material in 30 minutes at 37°C under standard assay conditions.
Quality Control: Exo-Minus Klenow DNA Polymerase (D355A, E357A) is free of detectable endo- and exonuclease and RNase activities.
References
- Feinberg, A.P. and Vogelstein, B. (1984) Anal. Biochem. 137, 266.
- Sanbrook, J. et al. (1989) In: Molecular Cloning: A Laboratory Manual (2nd ed.), Cold Spring Harbor Laboratory Press, New York. v.2, p. 8.14.
- Walter, N.G. (1995) J. Mol. Biol. 254, 856.
Ordering
Catalog No. | Concentration | Size |
---|
Exo-Minus Klenow DNA Polymerase (D355A, E357A)
KL11101K | 10 U/µl | 1,000 Units |
KL111K | 5 U/µl | 1,000 Units (4 x 250 U) |
KL11250 | 5 U/µl | 250 Units |
KL115K | 5 U/µl | 5,000 Units |
Exo-Minus Klenow DNA Polymerase
KL080911K | 10 U/µl | 1,000 Units |
KL105000 | 5 U/µl | 5,000 Units |