DNA Polymerase I, E. coli
THIS PRODUCT HAS BEEN DISCONTINUED
Applications
- Generation of labeled DNA probes by nick translation.1
- Second-strand cDNA synthesis.2
- In vitro synthesis of DNA.
DNA Polymerase I from E. coli is a DNA-dependent DNA polymerase. The enzyme also contains both 5´→3´ and 3´→5´ exonuclease activities.3 The 5´→3´ exonuclease activity enables the enzyme to use nicks and gaps in the DNA as starting points for labeling DNA by nick translation.
Unit Definition: One unit of DNA Polymerase I, E. coli, converts 10 nmol of dNTPs into acid-insoluble material in 30 minutes at 37°C under standard assay conditions.
Storage Buffer: 50% glycerol containing 50 mM Tris-HCl (pH 7.5), 0.1 M NaCl, 0.1 mM EDTA, 1 mM DTT, and 0.1% Triton® X-100.
Quality Control: DNA Polymerase I, E. coli, is free of detectable contaminating endonuclease and RNase activities.
References
- Meinkoth, J. and Wahl, G.M. (1987) Meth. Enzymol. 152, 91.
- Gubler, U. and Hoffmann, B.J. (1983) Nucleic Acids Res. 16, 1999.
- Lehman, I.R. (1981) The Enzymes 14, 16.
Note: Catalog items DP02250, DP021K, and DP0415K have been discontinued.
Ordering
| Catalog No. | Concentration | Size |
|---|
DNA Polymerase I, E. coli
| DP081025K | 10 U/µl | 2,500 Units |
| DP0810500 | 10 U/µl | 500 Units |