qScript™ microRNA Quantification System
The Quanta microRNA Technology
The qScript microRNA cDNA Synthesis Kit is suitable for use with total RNA or preparations pre-enriched for microRNAs. A poly(A) tail is added to microRNAs followed by cDNA synthesis using an adapter primer and qScript RT in a single-tube reaction. The resulting cDNA is ready for qPCR amplification with a Universal PCR Primer and your choice of microRNA Assays. This unique single-tube system provides ease-of-use and efficient cDNA synthesis for quantification of microRNAs with a high degree of sensitivity and specificity. PerfeCTa® SYBR Green SuperMixes ensure compatibility with any real-time thermal cycler.
Highlights:
- Log-fold greater sensitivity than other market leading kits
- Compatible with either total RNA or microRNA-enriched samples
- Linear assay performance over a broad range of input RNA
- Seamlessly profile hundreds of miRs from a single RT reaction
- Eliminates inefficient stem-loop priming and costly hydrolysis probes
Confidence in Your Results
Quanta’s technology allows inclusion of a minus-poly(A) polymerase control not available with other protocols. Each kit also includes a positive control for SNORD44 that may also be used as a calibration target.
Detection of Rare microRNAs
Quanta’s microRNA profiling system provides linear detection and quantification of microRNAs across total-RNA input levels spanning six orders of magnitude. This means microRNAs will be detected when present even when tissue is scarce or the microRNAs are rare.
Superior Sensitivity
Quanta’s microRNA profiling system yields superior results when compared to other leading microRNA quantification systems (Figure 3). The qScript microRNA cDNA Synthesis Kit, PerfeCTa microRNA Assays and PerfeCTa SYBR Green SuperMix form an integrated system that yields industry-leading results.
PerfeCTa® microRNA Assays & Controls
Quanta’s PerfeCTa® microRNA Assays are wet-validated SYBR Green assays and are specific to the microRNA sequences in miRbase. Each consists of a single microRNA-specific primer that works in conjunction with the Universal PCR Primer to form a robust and specific assay.. Each qScript microRNA cDNA Sytnthesis Kit contains both the Universal PCR Primer and Positive Control Primer, which are also available individually.