MaxPlax™ Lambda Packaging Extracts
Applications
- Packaging of lambda, cosmid, or fosmid DNA for construction of genomic libraries.
MaxPlax™ Lambda Packaging Extracts offer maximum in vitro packaging efficiencies of cos site-containing methylated or unmethylated DNA. Traditional lambda packaging extracts are derived from two complementary lysogenic E. coli strains, BHB2690 and BHB2688, as described by Hohn.1 MaxPlax Extracts use a restriction-free E. coli K-12 strain, NM759, in place of strain BHB2690.2 The sonicated extracts of NM759 are combined with extracts of BHB2688 to produce MaxPlax Extracts.
Each lot of MaxPlax Extracts is tested and guaranteed to maintain a packaging efficiency of greater than 1 x 109 pfu/µg of Control Ligated Lambda DNA for 1 year from the date of purchase if stored properly. A Certificate of Analysis stating actual packaging efficiency (pfu/µg DNA) is provided with each lot of MaxPlax Extracts.
Benefits
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References
- Hohn, E.G. (1979) Methods Enzymol. 68, 299.
- Gunther, E.G. et al. (1993) Nucleic Acids Res. 21, 3903.
- Fiandt, M. (2000) Epicentre Forum 7(3), 14.
Table 1. Packaging of methylated and unmethylated DNA using MaxPlax™ Lambda Packaging Extracts. | |
Cosmid Insert | Pfu/µg DNA |
Unmethylated DNA | 3.5 x 107 |
Methylated DNA | 3.9 x 107 |
Ordering
Catalog No. | Size |
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MaxPlax™ Lambda Packaging Extracts
MP5105 | 5 Extracts |
MP5110 | 10 Extracts |
MP5120 | 20 Extracts |