Fast-Link™ DNA Ligation Kit
Applications
The Fast-Link™ DNA Ligation Kit uses a high-quality ligase (Fast-Link T4 DNA Ligase), that was cloned at Epicentre and formulated to provide extremely rapid, high-efficiency DNA ligation. Cohesive-end ligations can be performed in 5 minutes at room temperature. In contrast to other ligases, it is not necessary to desalt Fast-Link ligation reactions prior to transformation of electrocompetent or chemically competent cells. The Fast-Link Kit can be used for routine and high-throughput DNA cloning. |
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Benefits
- Cohesive-end ligations in 5 minutes at room temperature (Fig. 1).
- Blunt-end ligations in 15 minutes at room temperature (Fig. 2).
- Ligation of PCR product with A-overhangs in 1 hour at 16°C.
- High ligation efficiency (Table 1).
- Desalting of ligation products is not needed prior to transformation of electrocompetent or chemically competent cells.
- BAC ligation with inserts >100 kb can be performed in 4 hours, instead of overnight reactions as with other ligases.
| Type of End | Ligation Time | % White Colonies | Recombinants per µg DNA |
| Overhang | 5 min. | 93 | 2.0 x 106 |
| Blunt | 15 min. | 71 | 4.4 x 105 |
| PCR product with 3´-A overhang | 1 hr. | 68 | 1.2 x 104 |
Table 1. Fast-Link™ representative results.
Figure 1. Time course for cohesive end ligation using the Fast-Link™ Kit. Lambda Hind III markers were ligated using 2 units of Fast-Link DNA Ligase (Lanes 2-6). Lane M, Kilobase ladder; lane 1, no enzyme.
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Figure 2. Time course for blunt-end ligation using the Fast-Link™ Kit. pUC 19 DNA digested with Pvu II was ligated using 2 units of Fast-Link DNA Ligase (lanes 1-6). Lanes M, Kilobase ladder.
Ordering
| Catalog No. | Size |
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Fast-Link™ DNA Ligation Kit
| LK0750H | 50 Ligations |
| LK6201H | 100 Ligations |