2´-Fluorine-2´-Deoxyribonucleoside-5´-Triphosphate Solutions

Epicentre’s NTPs are provided as sterile, neutral solutions of ATP, CTP, GTP, or UTP, each at either 10 mM or 100 mM, or as a premixed solution of all four NTPs at 2.5 mM each for use in RNA synthesis.

The ATP Solution is also suitable for use as a cofactor with T4 DNA Ligase for ligating DNA molecules, with T4 RNA Ligase for ligating RNA molecules, with T4 Polynucleotide Kinase for phosphorylating the 5´ ends of DNA and RNA, with Plasmid- Safe™ ATP-Dependent DNase for digesting linear DNA, and with Poly(A) Polymerase for tailing RNA.

2´-Fluorine-dCTP and 2´-Fluorine-dUTP are supplied as neutralized solutions at 50 mM each. These nucleotides are efficiently incorporated into RNA transcripts in vitro by Epicentre’s patented T7 R&DNA™ Polymerase.

Storage Buffer: NTP Solutions and 2´-F-dC/dU are made with sterile deionized water and adjusted to pH 7.0.

ATP (Adenosine-5´-Triphosphate) Formula (free acid): C10H16N5O13P3 Formula weight: 507 A250/A260 = 0.80 A280/A260 = 0.14	CTP (Cytidine-5´-Triphosphate) Formula (free acid): C9H16N3O14P3 Formula weight: 483 A250/A260 = 0.84 A280/A260 = 0.97	GTP (Guanosine-5´-Triphosphate) Formula (free acid): C10H16N5O14P 3 Formula weight: 523 A250/A260 = 1.14 A280/A260 = 0.65
UTP (Uridine-5´-Triphosphate) Formula (free acid): C9H15N2O15P3 Formula weight: 484 A250/A260 = 0.74 A280/A260 = 0.36	2´-Fluorine-dUTP (2´-F-dUTP) Formula: C9H10N2O14FP3Li4 MW: 509.86 Purity: > 90%	2´-Fluorine-dCTP (2´-F-dCTP) Formula: C9H11N3O13FP3Li4 MW: 508.88 Purity: > 90%

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