AmpliScribe™ T7-Flash™ Biotin-RNA Transcription Kit

Applications

Production of nonradioactive RNA probes for any purpose, including microarray (transcription profiling) studies, in situ hybridization, and blotting experiments.

The AmpliScribe™ T7-Flash™ Biotin-RNA Transcription Kit enables high-yield synthesis of biotin-RNA or biotin-antisense RNA (aRNA, also called cRNA). The kit incorporates Biotin-16-UTP directly into the RNA transcript. High yields of biotin-RNA are produced from as little as 50 ng of input DNA template.

Benefits

The kit uses Epicentre’s AmpliScribe T7-Flash rapid, high-yield in vitro transcription technology to generate the highest possible yield of biotin-RNA.
In vitro transcription reaction conditions are optimized to yield optimal levels of uniformly labeled biotin-RNA for high signal intensity.
RNase Inhibitor included.

		Incubation Time (minutes)
		30	60 (1 hour)	120 (2 hours)	480 (4 hours)
Template DNA	50 ng	5 – 10 µg	15 – 25 µg	30 – 40 µg	60 – 70 µg
	100 ng	15 – 20 µg	35 – 45 µg	70 – 80 µg	130 – 140 µg
	500 ng	60 – 70 µg	140 – 150 µg	> 180 µg	> 180 µg
	1.0 µg	125 – 135 µg	> 180 µg	> 180 µg	> 180 µg

Table 1. Yield of Biotin-RNA (in mg) from varying amounts of control template DNA from a standard 37°C, 20 ml AmpliScribe T7-Flash Biotin-RNA Transcription Kit reaction over time. Results may vary depending on the template used.

Ordering

Catalog No.			Size

AmpliScribe™ T7-Flash™ Biotin-RNA Transcription Kit

ASB71110

10 Reactions

Contents: AmpliScribe™ T7-Flash™ Enzyme Solution, RiboGuard™ RNase Inhibitor, AmpliScribe™ T7-Flash™ 10X Reaction Buffer, NTP/Biotin-UTP PreMix (ATP, CTP, GTP, UTP, and Biotin-16-UTP) Solution, RNase-Free Water, RNase-Free DNase I, DTT, Control DNA Template (linearized).