Kit Name |
End Product and Applications |
RNA Yield Per 1 µg of Control DNA template |
Reaction time |
Comments |
AmpliScribe™ T7 High Yield Transcription Kit |
High yields of RNA for many applications |
100 µg – 130 µg |
2 hours |
Epicentre’s original AmpliScribe Kit |
AmpliScribe™ T7-Flash™ Transcription Kit |
High yields of RNA for many applications |
150 µg – 180 µg |
30 minutes |
Produces more RNA in shorter reaction time than other commercial kits. |
AmpliScribe™ T7-Flash™ Biotin-RNA Transcription Kit |
Biotin-labeled RNA for use as non-radioactive RNA probe |
150 µg – 180 µg |
2 hours |
|
DuraScribe® T7 Transcription Kit |
DuraScript® RNA that is resistant to RNaseA and “finger nucleases”. Ideal for RNA Aptamer, ribozyme, RNAi, anti-sense RNA studies. |
30 µg – 50 µg |
2 – 6 hours |
Kit includes DuraScribe® T7 RNA Polymerase that incorporates 2´-F-dCTP and 2´-F-dUTP, as well as ATP and GTP into full length DuraScript RNA transcripts that are resistant to RNase A. |
MiniV™ In Vitro Transcription Kit |
RNA for many applications |
1 µmole – 5 µmole per 1 µmole of ssDNA template |
30 minutes |
In vitro transcription of RNA from single-strand DNA templates |
Poly(A) Polymerase Tailing Kit |
Adds a 3´ poly(A)-tail to in vitro transcribed RNA. |
|
30 – 60 minutes |
Includes Poly(A) Polymerase enzyme, Reaction Buffer and ATP. |
RiboGuard™ RNase Inhibitor |
|
|
|
Inhibits a variety of RNases including RNaseA. |