MessageBOOSTER™ cDNA Synthesis Kit for qPCR
Applications
The MessageBOOSTER™ cDNA Synthesis Kit for qPCR* enables the user to perform sensitive qPCR amplifications using the total RNA from very small populations of cells, even from as little as one cell (Table 1). The kit amplifies the mRNA contained in a small total RNA sample and then converts the amplified RNA to cDNA that is ready for PCR or qPCR (Fig. 1). The MessageBOOSTER cDNA Synthesis Kit for qPCR uses oligo(dT) to prime cDNA synthesis. Therefore, this kit is best used with intact total RNA preparations. Benefits
|
||
Figure 1 (click to enlarge). Overview of the MessageBOOSTER™ cDNA Synthesis Kit procedure. The kit uses a linear RNA amplification process to amplify the RNA from a small population of cells, then converts the amplified RNA to cDNA that is ready for PCR. The procedure can be completed in 1 day.
Figure 2. Sensitivity of the MessageBOOSTER™ cDNA Synthesis Kit. cDNA produced from 10 pg of total NRK RNA significantly improved the sensitivity (lowered the CT) of detecting the low-abundance PBGD transcript compared to cDNA produced from 10 pg of unamplified RNA.
|
Table 1. The number of qPCR amplifications that can be performed using cDNA produced by a MessageBOOSTER™ cDNA Synthesis Kit. The number of qPCRs is dependent on the amount of input total RNA and the abundance of the transcript(s) of interest. |
Low-Abundance Transcripts = 1-1,000 copies per cell Medium-Abundance Transcripts = 1,000-10,000 copies per cell High-Abundance Transcripts = 10,000-100,000 copies per cell |
Product Citations
- Grunenwald, H. et al. (2006) Epicentre Forum 13(3), 22.
- DeFazio, R.A. et al. (2006) J. Neuroscience 26, 3971.
- Kaeffer, B. et al. (2007) Pediatric Research 62, 564.
- Lochner, M., et al. (2008) J. Exp. Med. 205, 1381.
- Graham, D.M. et al. (2008) J. Neurophysiology, 99, 2522.
- Liu, X. et. al. (2009) Am. J. Physiol. Lung Cell Mol Physiol. 296, L158
- Yakovlev, I.A. et al. (2008) Fungal Genetic and Biology 45, 498
- Jung, Y. et al. (2008) Stem Cells Express 10, 1634
- Xi, D. et al. (2008) J. Neurosci Method 10, 1016
- Michel, M-L. et al. (2008) Proc. Nat’l Acad Sci-USA 105(50), 19845
- Satoh-Takayama, N. et al. (2008) Immunity 29, 958
- Bouskra, D. et al. (2008) Nature 10, 1038
- Yakovlev, I.A. et al. (2008) Fungal Genetics and Biology 45, 498
- Roberts, C.D. et. al. (2009) J. Physiology 587, 1657
- Peduto, L. et. al. (2009) J. Immunology 182, 5789
- Xi, D. et. al. (2009) Int’l J. Neuropsychopharmacology, May 13:1-14
*MessageBOOSTER Products are covered by intellectual property licensed to Epicentre Technologies Corporation from Johnson & Johnson Pharmaceutical Research & Development, L.L.C., and by intellectual property sublicensed to Epicentre Technologies Corporation from Incyte Corporation.
Ordering
Catalog No. | Size |
---|
MessageBOOSTER™ cDNA Synthesis Kit for qPCR
MB060124 | 24 Reactions |