TargetAmp™-Nano/Pico Labeling Kit for Illumina® Expression BeadChip®

TargetAmp™: Selection Guide | Process | Performance | FAQ | Poster (3 MB PDF)

The TargetAmp™-Nano Labeling Kit and TargetAmp™-Pico Labeling Kit produce biotin-aRNA (also called biotin-cRNA) that has been optimized for high signal intensity on Illumina Expression BeadChip arrays, enabling sensitive gene detection and reproducible differential expression results.

Benefits

TargetAmp™-Nano Labeling Kit for Illumina® Expression BeadChip®

• Fast. Perform the 6-hour reaction and begin BeadChip hybridization the same day.
• High Sensitivity. Sensitive and reproducible gene detection.
• High Yield. Make microgram amounts of biotin-aRNA (biotin-cRNA) from as little as 25 ng of total RNA.
• High Fidelity. TargetAmp-Nano labeling procedure preserves the relative abundance of the original sample.

TargetAmp™-Pico Labeling Kit for Illumina® Expression BeadChip®

• High Sensitivity. Sensitive and reproducible gene detection.
• High Yield. Make microgram amounts of biotin-aRNA (biotin-cRNA) from as little as 50 pg of total RNA.
• High Fidelity. TargetAmp-Pico labeling procedure preserves the relative abundance of the original sample.
• High correlation with TargetAmp-Nano Kit.
• Small Sample Transcription Profiling. Ideal for laser-captured, microdissected, flow-sorted and other small samples.

 

Table 1. TargetAmp-Nano Kit and TargetAmp-Pico Kit Selection Guide

	Input RNA	Reaction Time	Additional
TargetAmp-Nano Kit	25 ng – 500 ng total RNA	6 hours	Requires use of SuperScript III Reverse Transcriptase (provided by the user)
TargetAmp-Pico Kit	50 pg – 500 pg total RNA	2 days	Requires use of SuperScript III and Superscript II Reverse Transcriptases (provided by the user)

 

Table 2. Yield of Biotin-aRNA from TargetAmp-Nano Kit and TargetAmp-Pico Kit reactions

Input RNA (HeLa total RNA)	50 pg	100 pg	500 pg	25 ng	100 ng	500 ng
TargetAmp-Nano Kit reaction				3 μg	10 μg	75 μg
TargetAmp-Pico Kit reaction	8 μg	19 μg	71 μg

 

Figure 1 (click to enlarge). Genes detected using Biotin-aRNA made using the TargetAmp-Nano and TargetAmp-Pico Kits.

Figure 2 (click to enlarge). Mouse liver/skeletal muscle expression ratios were obtained using Biotin-aRNA produced from 100 ng of RNA using the TargetAmp-Nano Kit and from 100 pg of total RNA using the TargetAmp-Pico Kit.

Ordering

Catalog No.			Size

TAP120210	10 Reactions
TAP120224	24 Reactions