MasterPure™ Yeast DNA Purification Kit

Poster on rapid fungal DNA typing from ASM 2006 (2.3 MB)

Applications

Purification of DNA from yeast for many molecular biology applications, including PCR amplification, restriction endonuclease digestion, Southern blotting, and genomic library preparation.
Identification and typing of fungi.

The MasterPure™ Yeast DNA Purification Kit enables efficient, high-yield purification of high-molecular-weight DNA from yeast and other fungi. The protocol involves nonenzymatic cell lysis at 65°C, followed by removal of protein by precipitation, and nucleic acid precipitation and resuspension. No lyticase, proteolytic enzymes, or bead-beating are used in the procedure. Yeast genomic DNA yields using the MasterPure Kit are much higher than yields obtained with other commercially available kits (Fig. 1).¹ The protocol can be easily adjusted for larger or smaller samples, including single yeast colonies.¹ The recovered nucleic acid can be used directly in most applications, including PCR amplification.

Benefits

Higher yields of yeast chromosomal DNA than with other kits.
Recover DNA from a wide variety of yeast species, including Candida, Saccharomyces, Pichia, and Schizosaccharomyces, and filamentous fungi such as Aspergillus² and Penicillium.
Purify DNA in less than 40 minutes.
No enzymatic lysis, columns, or phenol or other organic extractions. No bead-beating.
The recovered DNA has a high molecular weight (Fig. 2), and is suitable for many applications, including PCR amplification.

References

Hoffman, L. and Moan, E. (1998) Epicentre Forum 5(4), 1.
Jin, J., Lee, Y.-K. and Wickes, B.L. (2004) J. Clin Microbiol. 42, 4293.

Figure 1. Comparative yields of DNA from the MasterPure™ Yeast DNA Purification Kit and competitor’s kits. DNA was quantitated specifically with Hoechst fluorescent dye 33258, which gives minimal fluorescence with RNA. The data represent the average DNA yields determined by fluorometry from two experiments with S. cerevisiae and C. albicans. The MasterPure Kit produced up to 17 times more DNA from C. albicans and 12 times more DNA from S. cerevisiae than other kits.

Figure 2. Size distribution of DNA produced from the MasterPure™ Kit. For each kit indicated, a 500-ng aliquot of purified yeast DNA was analyzed by pulse-field gel electrophoresis on a 1% agarose gel. The gel was stained with ethidium bromide. The majority of DNA isolated with the MasterPure Kit was estimated to be in the 40- to 50-kb size range, while degradation to smaller fragments was observed with other kits. Lane M, lambda DNA ladder; Lane 4, T7 DNA (40 kb).

Ordering

Catalog No.			Size

MasterPure™ Yeast DNA Purification Kit

MPY80010	10 Purifications
MPY80200	200 Purifications

Contents: Yeast Cell Lysis Solution, MPC Protein Precipitation Reagent, TE Buffer, RNase A.

Note: Catalogue number MPY80010 has been discontinued, effective from August 2014. This product is available only until stocks last.