QuickExtract™ Plant DNA Extraction Solution

Applications

High-throughput isolation of DNA from plant leaf samples for PCR-based analysis, e.g., GMO testing.

The QuickExtract™ Plant DNA Extraction Solution can be used to rapidly and efficiently extract PCR-ready genomic DNA from most plant leaf samples using a simple, one-tube protocol that takes only 8 minutes (Fig. 1). Most leafy plants are suitable for DNA extraction using the QuickExtract Plant Solution, including Arabidopsis, barley, maize, emmer, pepper, rice, spelt, spinach, soybeans, and wheat (e.g., Fig. 2).

The QuickExtract Plant method allows for the inexpensive processing of one to hundreds of samples simultaneously, without grinding the sample, centrifugation, spin columns, or any toxic organic solvent. The procedure is fully compatible with robotic automation, provides a PCR-ready sample, and is reproducible (Fig. 3). Simply add the QuickExtract Plant solution to the sample and perform two sequential heating steps. A small aliquot of the sample is then used as a template for PCR or qPCR.

Figure 1 (click to enlarge). Overview of the QuickExtract™ Plant DNA extraction procedure

Benefits

qPCR or PCR-ready sample.
No bead-beating, freezing, or grinding of plant leaf material.
Nontoxic, inexpensive processing.
Fast procedure (8 minutes for average sample).
No centrifugation or spin columns to reduce yields.
Suitable for high-throughput or automated workflows.

Figure 2. PCR products using QuickExtract™ Plant DNA Extraction Solution with different varieties of plant leaves. Forty cycles of RAPD were performed with DNA extracted from plant leaves using the QuickExtract Plant solution and the FailSafe™ PCR System. Lane M, 100-bp ladder; lane 1, pepper; lane 2, soybean; lane 3, spelt.

Figure 3. Reproducibility of PCR results with QuickExtract™ Plant DNA Extraction Solution from Arabidopsis thaliana leaves. Six individual punches of four Arabidopsis leaves were treated with QuickExtract Plant solution as described in the product information sheet. One microliter of the solution was used in a 25-µl PCR using the FailSafe™ PCR System and primers specifi c for the single-copy HSC70 chromosomal gene. Aliquots were analyzed on a 2% agarose gel and the DNA was visualized by staining with SYBR® Gold. Lanes 1-6, PCR products of extracted DNA samples; lane 7, no-leaf negative control; lane M, 100-bp DNA ladder. The expected amplicon is approximately 520 bp.

Ordering

Catalog No.			Size

QuickExtract™ Plant DNA Extraction Solution

QEP70750	50 ml (500 Extractions)
QEP80705	5 ml (50 Extractions)