T7 RNA Polymerase

• Synthesis of RNA for nucleic acid amplification methods or gene expression studies.
• RNA synthesized can be used as: a hybridization probe, antisense RNA, a ribozyme, a template forin vitrotranslation, a precursor mRNA for splicing or other processing studies, or to make dsRNA for RNA interference or gene silencing.

The T7 RNA Polymerase produces defined RNA byin vitrotranscription of dsDNA that is downstream of the specific RNA polymerase promoter. This enzyme preparation is highly purified, and exhibits excellent promoter specificity.

Unit Definition:One unit of RNA polymerase catalyzes the incorporation of 1 nmol of ribonucleoside triphosphate into RNA in 1 hour at 37°C under standard assay conditions using a DNA template with the appropriate T7 promoter.

Storage Buffer:50% glycerol containing 50 mM Tris-HCl (pH 7.5), 0.1 M NaCl, 1.0 mM DTT, 0.1 mM EDTA, and 0.1% Triton® X-100.

Quality Control:The T7 RNA polymerase is tested inin vitrotranscription reactions, and is free of detectable exo- and endonuclease and RNase activities, andE. coliRNA polymerase activity.

References

1. Davanloo, P.et al.(1984)Proc. Natl. Acad. Sci.USA81, 2035.
2. Eberwine, J. (1992)Proc. Natl. Acad. Sci. USA89, 3010.(Note:Use of T7 RNA Polymerase for this application may require a license.)
3. Stahl, S. J. and Zinn, K. (1981)J. Mol. Biol.148, 481.

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