PerfeCTa® Reference Gene Assays
There is no shortage of reference gene primer sequences out there. Yet, scientists kept telling us about their frustrations with errant controls, multiple PCR products and other issues when normalizing with housekeeping genes such as GAPDH, Actin and others.
We took on the challenge of designing SYBR Green reference gene primers to help out some customers and learned first-hand why it’s so difficult to design a decent assay: the genome is littered with what we affectionately term garbage DNA sequences that resemble reference gene sequences very closely. Additional challenges also existed: Intronic sequence was often in short supply, or not conveniently located near good primer sites. Amplicons often would have lots of secondary structure, killing PCR efficiency. SNPs plagued reference gene sequences, making reproducibility from sample to sample difficult. Suffice it to say a lot of hard work went into our 10 human reference gene designs, from sophisticated bioinformatics to pure muscle through iterative testing of many different oligos. The result – 10 reference gene assays deserving of the PerfeCTa name.
- Validated for PCR efficiency
- Reduce or eliminate gDNA amplification
- Single SYBR Green melting peak
PerfeCTa Reference Gene Assays are concentration normalized and stabilized in a ready-to-use aqueous buffer and they ship ambient. No rehydration is necessary. Simply dilute to desired concentration. We provide enough for 500 qPCR reactions at 25µl final volume.
Standard Curve Statistics
*Ct values of reactions with 1000 copies (3.3 ng) of human genomic DNA or 10 ng of cDNA. ND = not detected.
Ordering
ACTB PerfeCTa Reference Gene Assay Human ACTB (Beta-actin) Cat# 95121-250 500 x 20-µl rxns |
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B2M PerfeCTa Reference Gene Assay Human B2M (Beta-2-microglobulin) Cat# 95122-250 500 x 20-µl rxns |
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GAPDH PerfeCTa Reference Gene Assay Human GAPDH (Glyceraldehyde 3-phosphate dehydrogenase) Cat# 95123-250 500 x 20-µl rxns |
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GUSB PerfeCTa Reference Gene Assay Human GUSB (Beta-glucuronidase) Cat# 95124-250 500 x 20-µl rxns |
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HPRT1 PerfeCTa Reference Gene Assay Human HPRT1 (Hypoxanthine-guanine phosphoribosyltransferase) Cat# 95125-250 500 x 20-µl rxns |
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PPIA PerfeCTa Reference Gene Assay Human PPIA (Peptidylprolyl isomerase A (cyclophilin A)) Cat# 95126-250 500 x 20-µl rxns |
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TFRC PerfeCTa Reference Gene Assay Human TFRC (Transferrin receptor) Cat# 95127-250 500 x 20-µl rxns |
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UBC PerfeCTa Reference Gene Assay Human UBC (Ubiquitin C) Cat# 95128-250 500 x 20-µl rxns |
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YWHAZ PerfeCTa Reference Gene Assay Human YWHAZ (Tyrosine 3-monooxygenase / tryptophan 5-monooxygenase activation protein, zeta polypeptide) Cat# 95129-250 500 x 20-µl rxns |
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RPL13A PerfeCTa Reference Gene Assay Human RPL13A (60S ribosomal protein L13a) Cat# 95130-250 500 x 20-µl rxns |