PerfeCTa PreAmp 5X SuperMix
PerfeCTA PreAmp SuperMix allows high order multiplexed enrichment of specific sequences from limiting template using low-cycle PCR.
Features & Benefits:
- 5X concentrated SuperMix for lower reaction volumes / higher sample volumes
- Adds more value to your precious samples
- AccuStart II hot-start
PerfeCTa PreAmp SuperMix is a 5X concentrated, ready-to-use reaction cocktail for unbiased, selected enrichment of target sequences from limiting amounts of starting material for downstream gene expression profiling or targeted re-sequencing. It contains all components, except primers and templates. The 5X concentrated SuperMix allows addition of higher template volumes when working with low concentration samples, and/or reduced reaction volumes. Inclusion of an inert light blue tracer dye helps visualize small reaction volumes and ensure accurate pipetting. PerfeCta PreAmp SuperMix delivers unbiased pre-amplification of up to 100 target sequences from as little as 100 pg of total RNA. It is compatible with both TaqMan 5′-nuclease probes or ds-DNA binding dye (i.e. SYBR Green I) qPCR detection chemistries. A key component of PerfeCTa PreAmp SuperMix is an ultra pure, highly processive thermostable DNA polymerase that is combined with high avidity monoclonal antibodies. This proprietary polymerase mix is resistant to PCR inhibitors and provides an extremely stringent automatic hot-start allowing reaction assembly, and temporary storage, at room temperature prior to pre-amplification.
Performance Data
PreAmp Process Flow:
Contents
- 5X reaction buffer containing optimized concentrations of MgCl2, dNTPs (dATP, dCTP, dGTP, dTTP), hotstart DNA polymerase, AccuVue blue tracer dye, and stabilizers.
Storage conditions
PerfeCTa PreAmp SuperMix is stable for 1 year when stored in a constant temperature freezer at -20°C, protected from light. For convenience, it may be stored unfrozen at +2 to +8°C for up to 6 months. Repeated freezing and thawing of the supermix will not affect product performance.
Ordering
Cat# 95146-040 40 x 50 µl rxns
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