eQo 1-Step ToughMix®
Features & Benefits
- Unrivaled Sensitivity – Detection down to 2 copies of RNA
- Sustainable & Earth Friendly – Lyophilized eQo bead format eliminates dry ice, CO2 consumptions & reduces shipping costs
- Novel Reverse Transcriptase – Convenient, ambient reaction setup. Overcomes rigid RNA secondary structure
- High Stability – Up to 1 year at room temperature, up to 1 year rehydrated at -20°C
- Tough Tested – Broad and significantly enhanced tolerance to PCR inhibitors
eQo 1-Step ToughMix is intended for molecular biology applications. This product is not intended for the diagnosis, prevention or treatment of a disease.
Description
eQo 1-step ToughMix® is a lyophilized reagent system for reverse transcription quantitative PCR (RT-qPCR) of RNA templates using hybridization probe detection chemistries such as TaqMan® 5’-hydrolysis probes. It is supplied with a proprietary rehydration buffer that when combined with the lyophilized “eQo beads” produces a stabilized 4X concentrated master mix. The kit includes thermolabile UDG for amplicon carryover elimination, an enhanced warm-start reverse transcriptase (RT) and all other required components for 1-step RT-qPCR except RNA template and primer/probe(s). The reaction chemistry has been optimized for inhibitor tolerance and delivers exceptional performance in either single-plex or highly demanding multiplex 1 step RT-qPCR formats.
Storage & Handling:
Prior to rehydration:
Store all components refrigerated at 2°C to 8°C or at ambient temperature (≤ 25°C) until kit expiration date. Do not open the internal silver Master Mix pouch until ready to rehydrate the lyophilized beads using the included buffer. See below for the rehydration protocol.
For lot specific expiry date, refer to package label, Certificate of Analysis or Product Specification Form.
After rehydration:
Store the 4X-concentrated 1-step ToughMix at -20°C (-25°C to -15°C). The rehydrated ToughMix is stable at this temperature and withstands up to 20 freeze-thaw cycles without compromised functional performance. After thawing, mix gently, but thoroughly by vortexing. Centrifuge briefly to collect contents before use.
Performance Data:
