sparQ RNA-Seq HMR Kit

Ultra FAST RNA library prep with integrated rRNA & globin depletion

sparQ RNA-Seq HMR Kit simultaneously depletes rRNA and globin mRNA while generating stranded RNA-seq libraries for Illumina^® NGS platforms in 5 hours. In a single step and tube, RNA fragmentation and depletion of abundant ribosomal and globin transcripts (human, mouse, and rat) are integrated. The proprietary, highly optimized enzymes and streamlined workflow generate high quality, directional transcriptome NGS libraries from either intact or degraded RNA samples, with key improvements for low input and FFPE samples.
Features & Benefits

• High quality directional RNA library prep in 5 hours
• Simple workflow with 3 reaction tubes, 9 steps and 9 components
• Efficient and integrated removal of rRNA and globin mRNA from
  human/mouse/rat (HMR) samples
  Improved results for samples with limited quantity and/or poor quality RNA

sparQ RNA-Seq HMR Kit is intended for molecular biology applications. This product is not intended for the diagnosis, prevention or treatment of a disease.

sparQ UDI Adapter Resources 

•  Technical Note – Setting up sparQ Library Prep Kit in Illumina Experiment Manager
•  Illumina Experiment Manager version 1.8 files (zip-download)
•  Illumina Experiment Manager version 1.9 files (zip-download)
•  sparQ UDI Adapter sequence overview

Storage and Handling:

• Store the product in a constant temperature freezer at -25°C to -15°C upon receipt.
• Once the ‘UDI Dilution Buffer’ has been thawed for use, it should be stored at 2 – 8°C.
• For lot specific expiry date, refer to package label, Certificate of Analysis or Product Specification Form.

Performance Data:

Resources:

Flyers: