QScript lyo 1-step RT-qPCR - Gene Target Solutions

Qscript lyo 1-step is intended for molecular biology applications. This product is not intended for the diagnosis, prevention or treatment of a disease.

Description

Qscript lyo 1-step is a lyophilized single-reaction reagent optimized for highly sensitive and reproducible one-step RT-qPCR using hydrolysis probes. The reagent contains a hot-start thermo-stable polymerase, a genetically engineered reverse transcriptase as well as other components to ensure higher performance detection of up to 5 targets with maximum sensitivity and specificity. The enhanced stability of the freeze-dried master mix enables convenient shipping and storage at room temperature. The single tube reaction facilitates easy reaction set up while preventing potential cross-contamination.

Freeze-dried master mix
Easy-to-use workflow
Ship and store at ambient temperatures

Detect as low as 0.5 pg RNA)
Greater sensitivity vs leading market RT-qPCR (liquid mastermix)

Qscript lyo 1-step demonstrates higher sensitivity and fluorescence detection than a market-leading one-step RT-qPCR product (liquid). The figure shows an LDHA assay with 10-fold serial diluted universal human RNA (50 ng to 0.5 pg) as template. 20 μl of LDHA primer/probe was used to resuspend each lyosphere, followed by addition of 5 μl of RNA target. RT-qPCR was carried out on a QuantStudio 7 instrument with the following conditions: 50°C 15 min, 95°C 3 min, 40 cycles: 95°C 15 sec, 60°C 60 sec.

Wide dynamic range from 0.5 pg – 500 pg

Qscript lyo 1-step was tested with 12 serial dilutions of Human total RNA (4x from 1250 ng to 0.3 pg). 20 μl of LDHA primer/probe was used to resuspend each lyosphere, followed by addition of 5 μl of RNA target. RT-qPCR was carried out on a QuantStudio 7 instrument with the following conditions: 50°C 15 min, 95°C 3 min, 40 cycles: 95°C 15 sec, 60°C 60 sec.

Expand assay conditions and targets with reliable multiplexing detection

Qscript lyo 1-step was tested with a 5-plex assay with 7 serial dilutions of Human total RNA (4x from 50 ng to 30 pg). 20 μl of primers/probes was used to resuspend each lyosphere, followed by addition of 5 μl of RNA target. RT-qPCR was carried out on a QuantStudio 7 instrument with the following conditions: 50°C 15 min, 95°C 3 min, 40 cycles: 95°C 15 sec, 60°C 60 sec.

Contents:

Qscript lyo 1-step mastermix (lyosphere)

Storage & Handling:

Store the Qscript lyo 1-step in a constant temperature freezer at 4°C upon receipt.
The kit can also be stored at room temperature for up to 9 months.
For lot specific expiry date, refer to package label, Certificate of Analysis or Product Specification Form.

Instrument Capability:

Resources:

Ordering:

Cat # 95198-008: Qscript lyo 1-step, 8R
Cat # 95198-024: Qscripty lyo 1-steph, 24R