2nd July 2024

sparQ DNA Frag & Library Prep Kit – Robust enzymatic fragmentation & consistent results

  • Simple 2 step workflow employs a unique enzyme mix safeguarding samples from over fragmentation
  • Tunable and reproducible fragmentation profiles
  • Flexible generation of high quality libraries from 1 ng – 1 µg of input DNA
  • PCR-free workflows enabled from 100 ng
  • Minimized bias across challenging regions for improved sequencing results

sparQ DNA Frag & Library Prep Kit is intended for molecular biology applications. This product is not intended for the diagnosis, prevention or treatment of a disease.

The sparQ DNA Frag & Library Prep Kit optimizes the integration of enzymatic fragmentation into a two-step protocol for the streamlined construction of libraries for sequencing on Illumina® NGS platforms. A single-tube enzyme mix facilitates the combination of fragmentation and DNA polishing minimizing DNA over fragmentation while greatly simplifying library. The streamlined workflow can be completed in under 3 hours with minimal hands-on time and can accommodate DNA input amounts from 1 ng to 1000 ng. DNA fragmentation and DNA polishing enzymes work together generating fragment sizes that are tunable and reproducible based on reaction time. Double-stranded DNA molecules are fragmented followed by DNA polishing reactions where 5′-phosphorylated and 3′-dA-tailed DNA fragments suitable for direct ligation of sequencing adapters are generated without the need for an intervening cleanup step. The HiFi PCR Master Mix and Primer Mix allow unbiased amplification of fragments with appropriate adapters ligated to both ends. PCR-Free workflows are enabled from 100 ng of starting material.

Streamlined workflow

  • Enzyme mix integrates fragmentation and DNA polishing reactions for a streamlined workflow
  • Complete workflow in under 3 hours with minimal hands on time

Tunable fragmentation

  • Adjusting fragmentation time generates selective DNA sizes
  • Produce more usable library fragments in the target range with sharper size distribution curves

Fragmentation time course

  • Select fragment size and input DNA amount to determine reaction times
  • Optimize fragmentation tuning for your sample

Minimize bias and maintain genome diversity

  • Low fragmentation and amplification bias enables better coverage uniformity
  • Consistent coverage independent of input DNA amounts
  • PCR-Free sequencing data correlates well with mechanical shearing results

Quality sequencing metrics

  • Industry leading sequencing results
  • High mapped reads with minimal duplication rates
  • Ensured return on sequencing investment

sparQ Adapter Barcode Sets

sparQ Adapter Barcode Sets A and B are single index barcoded oligonucleotide adapters designed for use with the Quantabio sparQ DNA Library Prep Kits in the construction of libraries for Illumina NGS platforms. Each sparQ Adapter Barcode Set contains 12 single index barcodes for 96 rxns.

Contents:

sparQ DNA Frag & Library Prep Kit contains:

  • DNA Frag & Polishing Enzyme Mix (5X)
  • DNA Frag & Polishing Buffer (10X)
  • DNA Frag Enhancer Solution
  • DNA Ligase
  • DNA Rapid Ligation Buffer
  • HiFi PCR Master Mix (2X)
  • Primer Mix

Storage & Handling:
Store kit components in a constant temperature freezer at -25°C to -15°C upon receipt. For lot specific expiry date, refer to package label, Certificate of Analysis or Product Specification Form.

Resources:

Ordering:

    • 95194-024 sparQ DNA Frag & Library Prep Kit (24 rxns)
    • 95194-096 sparQ DNA Frag & Library Prep Kit (96 rxns)
    • 95193-A96 sparQ Adapter Barcode Set A 12 single index barcodes for 96 rxns
    • 95193-B96 sparQ Adapter Barcode Set B 12 single index barcodes for 96 rxns