Moxi Go II Next Gen Flow Cytometer
3 or 4 Parameter Next Gen Flow
Increase the pace of scientific discovery with ORFLO’s next generation Moxi Go II™ Next Gen Flow Cytometers. The system is equipped with two fluorescent channels (525/45nm and 561nm/LP ) for multi-parameter cell analysis. Every assay also delivers simultaneous cell count & volume determination, using single-use flow cell. This eliminates the hassle of traditional flow associated with cleaning, maintenance, clearing of clogs, cross contamination and occasionally replacement of bottles and tubes.
Furthermore the Moxi Go II™ use very little sample volume, 60ul’s allowing you to conserver precious, expensive sample, such as stem cells. Cell concentrations as low as 10,000 cells per ml are possible, which most experiments would enable a little as 5ul’s of sample diluted in 55ul’s of PBS.
The Moxi Go II™ can be utilized as an assay development instrument and many powerful cell based assays can easily be optimized and run on the system. These include:
- Cell surface immuno-labeling
- In-Cell Protein Quant
- CRISPR/Transfection Optimization Studies
- Up to 4 plex bead based ELISA’s using Orflo’s new on board MPX Relative Quantitation App
- In cell Western Blots
- Reactive Oxidation Species Experiments
- Calein AM studies
- Phagocytosis Analysis
- Mito Potential Experiements
The Moxi Go II™ comes standard with an ultra-intuitive, plug-and-play interface with free OS updates as long as you own the instrument. No prior flow cytometry experience is required you simply just plug and play.
How it Works:
The operating principle behind the Moxi GO II Flow Cytometers is a unique combination of Coulter-style cell size determination with simultaneous fluorescence detection. As cells flow single file through the microfabricated single-use flow cell the volume of each particle is measured at the exact same time as their primary fluorescence is measured using a 488nm (MXG002) solid state diode laser with and with the following emission filters – 525/45nm (e.g. FITC, GFP, Alexa 488) and 561nm/LP (e.g. PE, RFP). Thousands of cells are measured in the 10 second read time and the data are plotted in a gradient density scatter plot as Cell size (volume) vs. Fluorescence (PMT voltage). Gating is easily performed on the unit using a interactive touch display, and the resulting live/dead ratios are automatically calculated (depending on the app selected). The analyzed data can also be displayed as a two color size histogram. Total volumetric cell counts are automatically determined for each test by precisely measuring the volume of fluid being analyzed.
Data:
Data can be displayed on the unit in both a color density scatter plot and a two color size histogram. Simply drag gates using the intuitive touch display for instant live/dead ratio calculations and each of the gated volumetric cell counts (i.e., total population, live population, and dead population (Viability App). The mean cell volume for the gated populations is also automatically displayed on the unit. Results from each test are stored in the standard FCS 3:1 format and can be viewed using any flow cytometry analysis package. The actual Moxi Flow screenshots from each assay (dot plots and histograms) are also stored in bitmap format for use online. Hundreds of files can be stored on each Moxi GO and are easily transferred to a Mac or PC using USB on-the-go. No aditional software is required.
Specifications:
| id | MXG102 |
| Included Accessories | USB power cord, US style USB power adapter, and Type MF-S cassette pack |
| AC Power Type | 110 VAC |
| Applications | Mulitplexed Bead ELISA’s|In Cell Westerrns|In Cell Protein Quant|GFP| Gold Standard Cell Count and Viability|Mito Potential|ROS|Phagocytosis |
| Average Cell Diameter Range | 3 – 16 microns Type MF-S |
| Battery Type | Rechargeable 3.7 V, 4500 mAh lithium ion |
| Cassette Types | Type MF-S |
| Cell Particle Concentration Range | 10,000 – 1,000,000 cells/mL Type MF-S |
| Cell Types Tested | HEK-293|HeLa|PC12|CD3+T|CHO-K1|Cos-7|HepG2|Hybridoma|Jurkat E6-1|K562|MCF7|Mesenchymal SC|Monocyte|Mouse ESC| NIH 3T3| PBMC (cultured)|Red Blood Cells (RBC)|L5178y| C. albicans (Yeast)| S. cerevisiae Vin 13 (Yeast)|S. cerevisiae X5 (Yeast)|Wine Yeast (natural fermentaion)|S.cerevisiae (Baker’s Yeast|Safale US-05 Yeast| |
| Data Output Formats | FCS 3.1, screen shots (.bmp), CSV |
| Data Storage Capacity | 16Mb |
| Display Resolution | 800 x 480 color touchscreen |
| Excitation Wavelengths | 488nm |
| In British Units | 10 lbs |
| Intended Use Statement | For Research Use Only. Product is not for use in diagnostic procedures |
| Laser Color | Blue |
| Measurable Dynamic Range | 3 – 26 microns Type MF-S |
| Measurement Time | 10 seconds Type MF-S |
| MPICell Health Ratio Moxi Viability Index MVI | Yes (Size histogram only) |
| Number of Detection Channels Flow parameters | 2 color, 1 size, 1 forward extinction |
| Number of PMTs | 2 |
| Optical Detection Range | 525/45nm (e.g. FITC, GFP) and 561nm/LP (e.g. PE, RFP) |
| Particle Size Detection Method | Impedimetric (Coulter Principle) |
| Platform | Open platform: 561nm/LP (PI, PE, DS Red, Sytox Orange, 7 AAD, Nile Red, Rhodamine Red, Sun Coast Yellow, PE/Cy5), 525/45nm (FITC, GFP, Alexa Fluor 488nm, Calcein) |
| Pre-Programmed Tests | Mulitplexed Bead ELISA’s|In Cell Westerrns|In Cell Protein Quant|RFP|Gold Standard Cell Count and Viability|Mito Potential|ROS|Phagocytosis |
| Resolution Histogram Bins | 1000 |
| Sample Type | Beads|Cell Preparations |
| Sample Volume | 60 µL |
| Supported Connectivity | USB on-the-go |
| Useable Cell Volume | 14 – 9,202 fL Type MF-S |
| Weight | 4.53 kg |
Application Notes
- Distinguishing CD4+ T-Cell Activated and Resting Populations
- An application note describing the use of Orflo’s Moxi Flow System in Distinguishing CD4+ T-Cell Activated and Resting Populations
- Moxi GO II – Early Stage Apoptosis Monitoring with Annexin V and PI
- Rapid Apoptosis Monitoring Using Annexin V and PI using Orflo’s Moxi GO II – Next Generation Flow Cytometer
- Moxi GO II – Transfection Efficiency Monitoring
- Application Brief describing GFP Transfection Efficiency Monitoring with Orflo’s Moxi GO II – Next Generation Flow Cytometer
- Moxi GO II – Brewery Yeast Counts and Health Monitoring
- Monitoring Yeast Counts, Viability, and Metabolic Activity in Brewing with Orflo’s Moxi GO – Next Generation Flow Cytometer
- Moxi GO Intracellular Immunolabeling Application Note
- Intracellular Immunolabeling with Orflo’s Moxi GO – Next Generation Flow Cytometer
- Moxi GO – PBMC-Immunophenotyping Application Note
- Immunophenotyping (CD marker labeling) PBMC’s with Orflo’s Moxi GO – Next Generation Flow Cytometer
- Moxi GO – Mitochondrial Membrane Potential
- Monitoring Mitochondrial Membrane Potential with Orflo’s Moxi GO – Next Generation Flow Cytometer
Protocols
- Moxi GO II – System Check Bead Verification Procedure
- Verification procedure
- Moxi GO II – Cell Surface Immunolabeling – Staining Protocol
- Step by step instructions for antibody labeling and detection of cell surface markers with the Moxi GO II System
- Moxi GO II – Whole Blood – Intracellular Immunolabeling Protocol
- Step by step instructions for intracellular immunolabeling of peripheral blood using the Moxi GO II system.
- Moxi GO II – Whole Blood – Cell Surface Immunolabeling Protocol
- Step by step instructions for cell surface immunolabeling of peripheral blood using the Moxi GO II system.
- Moxi GO II Cell Health (Calcein-AM) and Viability (PI) – Staining Protocol
- Step by step instructions for staining with Calcein-AM and PI using the Moxi GO II system.
- Moxi GO II Apoptosis (FITC-Annexin V) and Viability (PI) – Staining Protocol
- Step by step instructions for staining with FITC- Annexin V and PI using the Moxi GO II system.
- Moxi GO II – MitoPotential Protocol
- Step by step protocol for processing whole blood for measuring mitochondrial potential using TMRE on the Moxi GO II instrument.
Brochure:
Quick Start Guide:
Technical Notes
- Anti-Human CD2 PE
- Staining of PMBC cells with Anti-Human CD2-PE (red) vs mIgG1k Isotype control (gray).
- Anti-Human CD3 PE
- Staining of PMBC cells with Anti-Human CD3-PE (red) vs mIgG2ak Isotype control (gray).
- Anti-Human CD4 PE
- Staining of PMBC cells with Anti-Human CD4-PE (red) vs mIgG2ak Isotype control (gray).
- Anti-Human CD8a PE
- Staining of PMBC cells with Anti-Human CD8-PE (red) vs mIgG1k Isotype control (gray).
- Anti-Human CD11b PE
- Staining of PMBC cells with Anti-Human CD11b-PE (red) vs mIgG1k Isotype control (gray).
- Anti-Human CD45 PE
- Staining of PMBC cells with Anti-Human CD45-PE (red) vs mIgG1,k Isotype control (gray).