PerfeCTa SYBR Green SuperMix, UNG, ROX

PerfeCTa® SYBR® Green SuperMix, UNG, ROX™ is a 2X concentrated, ready-to-use reaction cocktail that contains all components, except primers and template for real-time quantitative PCR on Applied Biosystems 7000, 7300, 7700, or 7900 instruments. The proprietary buffer and stabilizers have been optimized exclusively for SYBR® Green I qPCR to deliver maximum PCR efficiency, sensitivity, and robust fluorescent signal. Highly specific amplification is crucial to successful qPCR with SYBR® Green I technology because this dye binds to and detects any dsDNA generated during amplification. A key component of this supermix is AccuStart™ Taq DNA polymerase which contains monoclonal antibodies that bind to the polymerase and keep it inactive prior to the initial PCR denaturation step. Upon heat activation (2 minutes at 95°C), the antibodies denature irreversibly, releasing fully active, unmodified Taq DNA polymerase. This enables specific and efficient primer extension with the convenience of room temperature reaction assembly. Additionally, the dNTP mix in this SuperMix contains dUTP in place of dTTP. Inclusion of uracil-N-glycosylase (UNG) prevents amplification of carry-over contamination from previous dU-containing PCRs.

Features & Benefits

• Buffer optimized for SYBR® Green stability, ensures reliable performance
• High yield, high sensitivit
• Highly specific amplification
• Superior reproducibility and precision

Performance Data

Comparison to QuantiTect, GAPD

PerfeCTa SYBR Green SuperMix Comparison to QuantiTect SYBR Green PCR Kit (Qiagen), GAPD
Log-fold serial dilutions of qScript™ cDNA from HeLa cell total RNA (100 ng to 0.1 pg) were analyzed by SYBR Green qPCR following each manufacturers protocol. Averaged plots of triplicate qPCR reactions are shown.

Real-Time PCR Instrument Compatibility

• Applied Biosystems 5700
• Applied Biosystems 7000
• Applied Biosystems 7300
• Applied Biosystems 7700
• Applied Biosystems 7900
• Applied Biosystems 7900HT
• Applied Biosystems 7900 HT Fast
• Applied Biosystems StepOne™
• Applied Biosystems StepOnePlus™

Contents

• 2X reaction buffer containing optimized concentrations of MgCl2, dNTPs (dATP, dCTP, dGTP, dUTP), AccuStart Taq DNA Polymerase, UNG, SYBR Green I dye, ROX Reference Dye and stabilizers.

Storage conditions

PerfeCTa® SYBR Green SuperMix, UNG, ROX is stable for 1 year when stored in a constant temperature freezer at -20°C, protected from light. For convenience, it may be stored unfrozen at +2 to +8°C for up to 6 months. Repeated freezing and thawing of the supermix is not recommended. However, the product demonstrated no loss of performance after 20 freeze-thaw cycles or 2 months at +20°C.

Resources

• 95069 PerfeCTa SYBR Green Supermix,UNG, ROX Protocol
• 95069 PerfeCTa SYBR Green Supermix,UNG, ROX MSDS
• SYBR SuperMixes and FastMixes Product Brochure

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