3rd July 2024

PerfeCTa SYBR Green SuperMix

PerfeCTa SYBR Green SuperMix is a user-friendly, 2X concentrated reaction mix that simplifies setup and reduces errors with optimized reference dye and pre-blended AccuVue plate loading dye for visual confirmation of reagent addition and mixing. This proprietary buffer technology stabilizes a high concentration of SYBR Green I dye to ensure maximum optical signal with low abundance or small targets (such as microRNA). Successful detection with a non-specific, dsDNA intercalating dye requires precise target amplification as off-target primer elongation will contribute to overall fluorescent signal and lead to over-reported relative abundance values. This reagent is powered by a highly-processive, ultra-pure Taq DNA polymerase mutant with stringent, ultra-pure AccuStart™II antibody hot start technology that allows ambient room-temperature setup and maximal enzyme kinetics after rapid, irreversible denaturation at 95°C.

  • 2X concentrated master mixes with stabilized SYBR Green dye and exceptional temperature stability (≥30 days at 22°C) that withstand repetitive freeze-thaw (≥ 20X).
  • Maximum SYBR Green dye load for robust optical signal with small amplicons. · Supports efficient vortexmixing with proprietary anti-foaming technology.
  • Maximum assay sensitivity and target precision with highly modified Taq DNA polymerase and stringentultrapure, AccuStart™ II antibody hotstart technology.
  • Easy-to-use with AccuVue™ plate loading dye and pre-blended passive reference dye.

Performance Data

Comparison to QuantiTect, GAPD

PageW - Graph Quanta vs Qiagen SYBR** - Quanta vs Qiagen SYBR

PerfeCTa SYBR Green SuperMix Comparison to QuantiTect SYBR Green PCR Kit (Qiagen), GAPD
Log-fold serial dilutions of qScript™ cDNA from HeLa cell total RNA (100 ng to 0.1 pg) were analyzed by SYBR Green qPCR following each manufacturers protocol. Averaged plots of triplicate qPCR reactions are shown.

Features & Benefits

  • Buffer optimized for SYBR® Green stability, ensures reliable performance
  • High yield, high sensitivity
  • Highly specific amplification
  • Superior reproducibility and precision

Real-Time PCR Instrument Compatibility

instrument capability

Contents

  • Single-tube, 2X concentrated reagent containing:
    • Reaction buffer with optimized concentrations of molecular-grade MgCl2, dATP, dCTP, dGTP, and dTTP.
    • AccuStart II Taq DNA Polymerase
    • SYBR Green I dye
    • Inert AccuVue dye
    • Proprietary enzyme stabilizers and performance-enhancing additives.
    • Titrated reference dye (if applicable).

Storage conditions

PerfeCTa SYBR Green SuperMix is stable for 1 year when stored in a constant temperature freezer at -20°C, protected from light. For convenience, it may be stored unfrozen at 4°C for up to 6 months, protected from light. After thawing, mix thoroughly before using. Stabilized reagent demonstrates no functional loss of performance after 20 freeze-thaw cycles or 2 months at 20°C.

Resources

Ordering

Cat# 95054-100 100 x 50-µl rxns (2 x 1.25 ml)
Cat# 95054-500 500 x 50-µl rxns (10 x 1.25 ml)
Cat# 95054-02K 2000 x 50-µl rxns (1 x 50 ml)