qScript™ One-Step Fast MGB qRT-PCR Kit, ROX™

Quanta BioSciences introduces the first One-Step qRT-PCR kits that overcome challenges of using MGB TaqMan probes for highly sensitive detection and quantification of RNA targets. qScript™One Step Fast MGB qRT-PCR kits set a new standard for high efficiency qRT-PCR results with a significantly faster cycling protocol for increased productivity. qScript™One-Step Fast MGB qRT-PCR Kits have been specifically optimized to provide exceptional qRT-PCR efficiency and sensitivity with TaqMan-MGB probes, such as TaqMan Gene Expression Assays from Applied Biosystems. Rapid cDNA synthesis and PCR kinetics deliver high performance qRT-PCR results in as little as 38 minutes. These kit are suitable for use in duplexed one-step qRT-PCR with VIC™-MGB labeled TaqMan Endogenous Controls,and other TaqMan MGB probes The qScript™One-Step Fast qRT-PCR Kits are available for all Real Time instrumentation platforms including those requiring normalization with ROX™reference dye. These kits are provided in 4X concentrated SuperMix format to enable analysis of higher volume of samples containing low abundance targets such as viral RNA.

Features & Benefits

• Flexible – Use Fast or standard qPCR cycling Conditions
• Optimized for MGB Probes- Get more sensitive and accurate results with Assay-on-Demand™ Primer/Probe sets
• No additional primer/probe optimization required for Fast cycling – Achieve the same, reliable results regardless of cycling conditions
• Broad dynamic range- Reliable data from your precious samples every time
• Fast protocol- Results in less than 40 minutes
• Concentrated 4X SuperMix- allows analysis of higher sample volume for rare targets

 Real-Time PCR Instrument Compatibility

• Applied Biosystems 5700
• Applied Biosystems 7000
• Applied Biosystems 7300
• Applied Biosystems 7700
• Applied Biosystems 7900
• Applied Biosystems 7900HT
• Applied Biosystems 7900 HT Fast
• Applied Biosystems StepOne™
• Applied Biosystems StepOnePlus™

Contents

• qScript One-Step Reverse Transcriptase – Optimized 20X formulation of recombinant MMLV reverse transcriptase for one-step Fast RT-PCR
• One-Step Master Mix, ROX (4X) – 4X reaction buffer containing dNTPs, magnesium chloride, AccuStart Taq DNA polymerase, ROX reference dye, and stabilizers
• Nuclease Free-Water

Storage conditions

Kit components are stable for one year when stored in a constant temperature freezer at -20°C. For convenience, the One-Step Master Mix may be stored unfrozen at +2 to +8°C for up to 6 months. Repeated freezing and thawing of the reaction mix is not recommended.

Resources

• 95083 qScript One-Step Fast MGB qRT-PCR kit ROX Protocol
• 95083 qScript One-Step Fast MGB qRT-PCR kit ROX MSDS

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