3rd July 2024

PerfeCTa MultiPlex qPCR ToughMix

PerfeCTa MultiPlex qPCR ToughMix is a 5X concentrated, ready-to-use reaction cocktail for real-time quantitative PCR (qPCR) that overcomes many known inhibitors of PCR often present in crude samples extracted from environmental specimens, plant tissues, or animal tissues. It contains all components, except primers, probes and templates. The 5X concentrated ToughMix allows addition of higher amounts of template and improved detection sensitivity with low concentration samples. PerfeCta MultiPlex qPCR ToughMix has been optimized to deliver maximum PCR efficiency, sensitivity, and specificity in reduced reaction volumes with fast cycle or conventional PCR cycling protocols.The system transcends multiplex limitations of conventional PCR master mixes, enabling unbiased amplification of up to five target sequences in a single tube. Suppression of low copy amplicons by high copy reference targets in the amplification is a common problem in multiplex PCR that can skew, or mask the apparent representation and quantification of low copy target sequences. PerfeCTa MultiPlex qPCR ToughMix results in multiplexed qPCR with dynamic range and sensitivity that are comparable to single-plex qPCR probe assays without the need for limiting or variable primer concentrations.A key component of PerfeCTa MultiPlex qPCR ToughMix is an ultra pure, highly processive thermostable DNA polymerase that is combined with high avidity monoclonal antibodies. This proprietary polymerase mix is highly resistant to PCR inhibitors and provides an extremely stringent automatic hot-start allowing reaction assembly, and temporary storage, at room temperature prior to PCR amplification.

Performance Data

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4-Plex Bias vs. 1-Plex
4-Plex Bias vs. 1-Plex

Real-Time PCR Instrument Compatibility

  • Roche LightCycler™ 480
  • Qiagen/Corbett Rotor-Gene® Q
  • Qiagen/Corbett Rotor-Gene® 6000
  • Qiagen/Corbett Rotor-Gene® 3000
  • Eppendorf Mastercycler® ep realplex
  • Eppendorf Mastercycler® ep realplex2 s
  • Cepheid SmartCycler®
  • Bio-Rad CFX96™
  • Bio-Rad CFX384™
  • Bio-Rad/MJ Opticon®
  • Bio-Rad/MJ Opticon 2
  • Bio-Rad/MJ Chromo4™
  • Bio-Rad MiniOpticon™
  • Illumina Eco qPCR
  • Thermo Scientific PikoReal Cycler
  • BioRad iCycler iQ™
  • BioRad MyiQ™
  • BioRad iQ™5

Contents

  • PerfeCTa Multiplex qPCR ToughMix 5X reaction buffer containing optimized concentrations of MgCl2, dNTPs (dATP, dCTP, dGTP,
    dTTP), hot-start DNA polymerase, and stabilizers.

Storage conditions

PerfeCTa Multiplex qPCR ToughMix is stable for 2 years when stored in a constant temperature freezer at -20°C, protected from light. For convenience, it may be stored unfrozen at +2 to +8°C for up to 6 months. Repeated freezing and thawing of the ToughMix will not affect product performance.

Precautions and Disclaimer

For research use only. Not intended for any animal or human therapeutic or diagnostic use.
Please consult the Material Safety Data Sheet for information regarding hazards and safe handling practices.

Ordering

Cat# 95147-250 250 x 25µL reactions (1 x 1.25 mL)
Cat# 95147-01K 1000 x 25µL reactions (4 x 1.25 mL)
Cat# 95147-05K 5000 x 25µL reactions (1 x 25 mL)