TransforMax™ EC100™ Chemically Competent E. coli
Applications
The highly versatile TransforMax™ EC100™ E. coli competent cells are ideal for most cloning applications. The cells provide very high transformation efficiency when tested against a wide range of supercoiled DNAs as well as DNA directly from a ligation reaction (Table 1). Benefits
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Genotype
F– mcrA Δ(mrr-hsdRMS-mcrBC) Φ80dlacZΔM15 ΔlacX74 recA1 endA1 araD139 Δ(ara, leu)7697 galU galK λ– rpsL (StrR) nupG
TransforMax EC100 Electrocompetent E. coli
- Transformation efficiency of >1 x 1010 cfu/µg of pUC19.
TransforMax EC100 Chemically Competent E. coli
- Transformation efficiency of >5 x 108 cfu/µg of pUC19.
DNA | TransforMax™ EC100™ Chemically Competent E. coli | TransforMax™ EC100™ Electrocompetent E. coli |
pUC19 | 1.4 x 108 | 1.4 x 1010 |
8.1-kb Clone | 1.3 x 107 | Not tested |
13.1-kb Clone | 4.3 x 106 | 1.3 x 109 |
23.1-kb Clone | 9.2 x 105 | 3.0 x 108 |
145-kb BAC Clone | Not tested | 7 x 107 |
13.1-kb clone directly from a ligation reaction | 2.2 x 105 | 2.1 x 107 |
Table 1. Comparison of the transformation efficiencies of TransforMax™ EC100™ E. coli with a variety of DNAs. Transformations were performed using 50 µl of competent cells and either supercoiled DNAs of the indicated sizes or a 1-µl aliquot from a standard 10-µl ligation reaction. Results shown are in cfu/µg of DNA and are the average transformation efficiencies obtained from several trials.
Ordering
Catalog No. | Size |
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TransforMax™ EC100™ Electrocompetent E. coli
EC10010 | 10 x 100 µl |
TransforMax™ EC100™ Chemically Competent E. coli
CC02810 | 10 x 50 µl |