RiboGuard- RNase Inhibitor

• Effective inhibition of the degradation of RNA by eukaryotic RNases in a variety of applications, including cDNA synthesis, RT-PCR, andin vitrotranscription and translation.

RiboGuard™ RNase Inhibitor is the best defense against common RNases, including RNase A, RNase B, and RNase C. This recombinant RNase inhibitor protein provides reliable protection of valuable RNA samples by binding strongly to RNases in a 1:1 ratio. Advanced purification protocols and extensive quality control tests ensure RiboGuard RNase Inhibitor is free of unwanted contaminants that can plague other commercially available preparations of RNase inhibitors.

Benefits

• A potent affinity for RNases (Ki>10-14M) ensures rapid inhibition even when trace amounts of RNase are present.
• Free of detectable RNase or DNase activity, and mammalian DNA.
• Does not interfere with enzymes commonly used to prepare or analyze RNA.
• Less sensitive to oxidation than traditional RNase inhibitors.

Unit Definition:One unit of RiboGuard RNase Inhibitor results in 50% inhibition of 5 ng of RNase A. Activity is measured by the inhibition of hydrolysis of 2,3´-cyclic monophosphate by RNase A.

Storage Buffer:50% glycerol solution containing 50 mM Tris-HCl (pH 7.5), 0.1 mM EDTA, 100 mM NaCl, and 10 mM DTT.

Quality Control:RiboGuard RNase Inhibitor is free of detectable DNA exo- and endonuclease, and RNase activities.

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