PerfeCTa SYBR Green SuperMix, ROX

PerfeCTa® SYBR® Green SuperMix, ROX™ is a 2X concentrated, ready-to-use reaction cocktail that contains all components, except primers and template for real-time quantitative PCR on Applied Biosystems 7000, 7300, 7700, or 7900 instruments. The proprietary buffer and stabilizers have been optimized exclusively for SYBR® Green I qPCR to deliver maximum PCR efficiency, sensitivity, and robust fluorescent signal. This supermix provides the highest level of specificity to reduce the occurrence or delay the detection of primer-dimer and other non-specific artifacts. Highly specific amplification is crucial to successful qPCR with SYBR® Green I technology because this dye binds to and detects any dsDNA generated during amplification. A key component of this supermix is AccuStart™ Taq DNA polymerase which contains monoclonal antibodies that bind to the polymerase and keep it inactive prior to the initial PCR denaturation step. Upon heat activation (2 minutes at 95°C), the antibodies denature irreversibly, releasing fully active, unmodified Taq DNA polymerase. This enables specific and efficient primer extension with the convenience of room temperature reaction assembly.

• 2X concentrated master mixes with stabilized SYBR Green dye and exceptional temperature stability (≥30 days at 22°C) that withstand repetitive freeze-thaw (≥ 20X).
• Maximum SYBR Green dye load for robust optical signal with small amplicons. · Supports efficient vortexmixing with proprietary anti-foaming technology.
• Maximum assay sensitivity and target precision with highly modified Taq DNA polymerase and stringentultrapure, AccuStart™ II antibody hotstart technology.
• Easy-to-use with AccuVue™ plate loading dye and pre-blended passive reference dye.

Performance Data

Comparison to QuantiTect, GAPD

PerfeCTa SYBR Green SuperMix Comparison to QuantiTect SYBR Green PCR Kit (Qiagen), GAPD
Log-fold serial dilutions of qScript™ cDNA from HeLa cell total RNA (100 ng to 0.1 pg) were analyzed by SYBR Green qPCR following each manufacturers protocol. Averaged plots of triplicate qPCR reactions are shown.

Features & Benefits

• Buffer optimization for SYBR® Green stability, ensuring reliable performance
• High yield, high sensitivity
• Highly specific amplification
• Improved reproducibility and assay precision

Real-Time PCR Instrument Compatibility

Contents

• Single-tube, 2X concentrated reagent containing:
  • Reaction buffer with optimized concentrations of molecular-grade MgCl2, dATP, dCTP, dGTP, and dTTP.
  • AccuStart II Taq DNA Polymerase
  • SYBR Green I dye
  • Inert AccuVue dye
  • Proprietary enzyme stabilizers and performance-enhancing additives.
  • Titrated reference dye (if applicable).

Storage conditions

PerfeCTa® SYBR Green SuperMix, ROX is stable for 1 year when stored in a constant temperature freezer at -20°C, protected from light. For convenience, it may be stored unfrozen at +2 to +8°C for up to 6 months. After thawing, mix thoroughly before using. Repeated freezing and thawing of the supermix is not recommended. However, the product demonstrated no loss of performance after 20 freeze-thaw cycles or 2 months at +20°C.

Resources

• 95055 PerfeCTa SYBR Green Supermix, ROX Protocol
• 95055 PerfeCTa SYBR Green Supermix, ROX MSDS
• SYBR SuperMixes and FastMixes Product Brochure

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